Cargando…

CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus

Perkinsus marinus (Perkinsozoa), a close relative of apicomplexans, is an osmotrophic facultative intracellular marine protozoan parasite responsible for “Dermo” disease in oysters and clams. Although there is no clinical evidence of this parasite infecting humans, HLA-DR4(0) transgenic mice studies...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yadavalli, Raghavendra, Umeda, Kousuke, Waugh, Hannah A., Tracy, Adrienne N., Sidhu, Asha V., Hernández, Derek E., Fernández Robledo, José A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062965/ https://www.ncbi.nlm.nih.gov/pubmed/33898400 http://dx.doi.org/10.3389/fbioe.2021.623278

Ejemplares similares

Responses of an oyster host (Crassostrea virginica) and its protozoan parasite (Perkinsus marinus) to increasing air temperature
por: Malek, Jennafer C., et al.
Publicado: (2018)

Assessment of Infection Prevalence and Intensity of Disease-Causing Parasitic Protozoans Perkinsus marinus and Haplosporidium nelsoni in Georgia Oysters
por: Batchelor, Sarah, et al.
Publicado: (2023)

The Alveolate Perkinsus marinus: Biological Insights from EST Gene Discovery
por: Joseph, Sandeep J, et al.
Publicado: (2010)

Humanized HLA-DR4 Mice Fed with the Protozoan Pathogen of Oysters Perkinsus Marinus (Dermo) Do Not Develop Noticeable Pathology but Elicit Systemic Immunity
por: Wijayalath, Wathsala, et al.
Publicado: (2014)

Identification of MMV Malaria Box Inhibitors of Perkinsus marinus Using an ATP-Based Bioluminescence Assay
por: Alemán Resto, Yesmalie, et al.
Publicado: (2014)

An Agar-Based Method for Plating Marine Protozoan Parasites of the Genus Perkinsus
por: Cold, Emma R., et al.
Publicado: (2016)

Development of a novel electroporation method for the oyster parasite Perkinsus marinus
por: Sakamoto, Hirokazu, et al.
Publicado: (2022)

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins
por: Soares Medeiros, Lia Carolina, et al.
Publicado: (2017)

Effects of salinity and temperature on in vitro cell cycle and proliferation of Perkinsus marinus from Brazil
por: QUEIROGA, FERNANDO RAMOS, et al.
Publicado: (2016)

Understanding Crassostrea virginica tolerance of Perkinsus marinus through global gene expression analysis
por: Proestou, Dina A., et al.
Publicado: (2023)

Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
por: Haldrup, Jakob, et al.
Publicado: (2023)

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
por: Zhang, Song, et al.
Publicado: (2021)

Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions
por: Chan, Jiulin, et al.
Publicado: (2021)

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
por: Svitashev, Sergei, et al.
Publicado: (2016)

In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein
por: Mehravar, Maryam, et al.
Publicado: (2019)

Investigation into the Physiological Significance of the Phytohormone Abscisic Acid in Perkinsus marinus, an Oyster Parasite Harboring a Nonphotosynthetic Plastid
por: Sakamoto, Hirokazu, et al.
Publicado: (2016)

Genetic signature analysis of Perkinsus marinus in Mexico suggests possible translocation from the Atlantic Ocean to the Pacific coast of Mexico
por: Ek-Huchim, Juan Pablo, et al.
Publicado: (2017)

Off-target effects in CRISPR/Cas9 gene editing
por: Guo, Congting, et al.
Publicado: (2023)

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus
por: Foster, Andrew J., et al.
Publicado: (2018)

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells
por: Hildreth, Andrew D., et al.
Publicado: (2020)

Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
por: Lee, Nahyun, et al.
Publicado: (2022)

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein
por: Bevacqua, Romina J., et al.
Publicado: (2023)

CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei
por: Zhou, Yajuan, et al.
Publicado: (2020)

A rapid phenotype change in the pathogen Perkinsus marinus was associated with a historically significant marine disease emergence in the eastern oyster
por: Carnegie, Ryan B., et al.
Publicado: (2021)

The application of CRISPR /Cas mediated gene editing in synthetic biology: Challenges and optimizations
por: Li, Wenqian, et al.
Publicado: (2022)

Extensive frameshift at all AGG and CCC codons in the mitochondrial cytochrome c oxidase subunit 1 gene of Perkinsus marinus (Alveolata; Dinoflagellata)
por: Masuda, Isao, et al.
Publicado: (2010)

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins
por: Malnoy, Mickael, et al.
Publicado: (2016)

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
por: Chaverra-Rodriguez, Duverney, et al.
Publicado: (2018)

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
por: Sant’Ana, Rodrigo Ribeiro Arnt, et al.
Publicado: (2020)

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing
por: Wei, Tuo, et al.
Publicado: (2020)

Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method
por: Yu, Lu, et al.
Publicado: (2022)

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes
por: Sinha, Tulika, et al.
Publicado: (2022)

CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila
por: Hu, Mengyi, et al.
Publicado: (2023)

CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference
por: Zhao, Yu, et al.
Publicado: (2020)

Identification of glycine betaine as a host-derived molecule required for the vegetative proliferation of the protozoan parasite Perkinsus olseni
por: Liu, Yuqi, et al.
Publicado: (2023)

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
por: Liang, Zhen, et al.
Publicado: (2017)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
por: Liu, Qizhi, et al.
Publicado: (2018)

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
por: Fu, Ya-Wen, et al.
Publicado: (2021)

Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma
por: Cui, Haodong, et al.
Publicado: (2021)

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases
por: Zhang, Yingxiao, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_qa7h8jnqp7o4ou7nrl2uanfm8j