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Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode

Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding lectin from the basidiomycete C. cinerea that is toxic to the bacterivorous nematode Caenorhabditis elegans as well as animal-parasitic and fungivorous nematodes. We expressed CCL2 in Arabidopsis to assess its protective potential toward plan...

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Autores principales: Moradi, Aboubakr, El-Shetehy, Mohamed, Gamir, Jordi, Austerlitz, Tina, Dahlin, Paul, Wieczorek, Krzysztof, Künzler, Markus, Mauch, Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063123/
https://www.ncbi.nlm.nih.gov/pubmed/33897746
http://dx.doi.org/10.3389/fpls.2021.657451
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author Moradi, Aboubakr
El-Shetehy, Mohamed
Gamir, Jordi
Austerlitz, Tina
Dahlin, Paul
Wieczorek, Krzysztof
Künzler, Markus
Mauch, Felix
author_facet Moradi, Aboubakr
El-Shetehy, Mohamed
Gamir, Jordi
Austerlitz, Tina
Dahlin, Paul
Wieczorek, Krzysztof
Künzler, Markus
Mauch, Felix
author_sort Moradi, Aboubakr
collection PubMed
description Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding lectin from the basidiomycete C. cinerea that is toxic to the bacterivorous nematode Caenorhabditis elegans as well as animal-parasitic and fungivorous nematodes. We expressed CCL2 in Arabidopsis to assess its protective potential toward plant-parasitic nematodes. Our results demonstrate that expression of CCL2 enhances host resistance against the cyst nematode Heterodera schachtii. Surprisingly, CCL2-expressing plants were also more resistant to fungal pathogens including Botrytis cinerea, and the phytopathogenic bacterium Pseudomonas syringae. In addition, CCL2 expression positively affected plant growth indicating that CCL2 has the potential to improve two important agricultural parameters namely biomass production and general disease resistance. The mechanism of the CCL2-mediated enhancement of plant disease resistance depended on fucoside-binding by CCL2 as transgenic plants expressing a mutant version of CCL2 (Y92A), compromised in fucoside-binding, exhibited wild type (WT) disease susceptibility. The protective effect of CCL2 did not seem to be direct as the lectin showed no growth-inhibition toward B. cinerea in in vitro assays. We detected, however, a significantly enhanced transcriptional induction of plant defense genes in CCL2- but not CCL2-Y92A-expressing lines in response to infection with B. cinerea compared to WT plants. This study demonstrates a potential of fungal defense lectins in plant protection beyond their use as toxins.
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spelling pubmed-80631232021-04-24 Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode Moradi, Aboubakr El-Shetehy, Mohamed Gamir, Jordi Austerlitz, Tina Dahlin, Paul Wieczorek, Krzysztof Künzler, Markus Mauch, Felix Front Plant Sci Plant Science Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding lectin from the basidiomycete C. cinerea that is toxic to the bacterivorous nematode Caenorhabditis elegans as well as animal-parasitic and fungivorous nematodes. We expressed CCL2 in Arabidopsis to assess its protective potential toward plant-parasitic nematodes. Our results demonstrate that expression of CCL2 enhances host resistance against the cyst nematode Heterodera schachtii. Surprisingly, CCL2-expressing plants were also more resistant to fungal pathogens including Botrytis cinerea, and the phytopathogenic bacterium Pseudomonas syringae. In addition, CCL2 expression positively affected plant growth indicating that CCL2 has the potential to improve two important agricultural parameters namely biomass production and general disease resistance. The mechanism of the CCL2-mediated enhancement of plant disease resistance depended on fucoside-binding by CCL2 as transgenic plants expressing a mutant version of CCL2 (Y92A), compromised in fucoside-binding, exhibited wild type (WT) disease susceptibility. The protective effect of CCL2 did not seem to be direct as the lectin showed no growth-inhibition toward B. cinerea in in vitro assays. We detected, however, a significantly enhanced transcriptional induction of plant defense genes in CCL2- but not CCL2-Y92A-expressing lines in response to infection with B. cinerea compared to WT plants. This study demonstrates a potential of fungal defense lectins in plant protection beyond their use as toxins. Frontiers Media S.A. 2021-04-09 /pmc/articles/PMC8063123/ /pubmed/33897746 http://dx.doi.org/10.3389/fpls.2021.657451 Text en Copyright © 2021 Moradi, El-Shetehy, Gamir, Austerlitz, Dahlin, Wieczorek, Künzler and Mauch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Moradi, Aboubakr
El-Shetehy, Mohamed
Gamir, Jordi
Austerlitz, Tina
Dahlin, Paul
Wieczorek, Krzysztof
Künzler, Markus
Mauch, Felix
Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title_full Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title_fullStr Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title_full_unstemmed Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title_short Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode
title_sort expression of a fungal lectin in arabidopsis enhances plant growth and resistance toward microbial pathogens and a plant-parasitic nematode
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063123/
https://www.ncbi.nlm.nih.gov/pubmed/33897746
http://dx.doi.org/10.3389/fpls.2021.657451
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