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Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells
OBJECTIVE: We investigated whether a previously reported association of IFNGR expression with rheumatoid arthritis (RA) and its radiographic severity reflects differences in proximal interferon‐γ (IFN‐γ) signaling in T cells from patients with RA compared with healthy controls (HC). METHODS: Using p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063148/ https://www.ncbi.nlm.nih.gov/pubmed/33779079 http://dx.doi.org/10.1002/acr2.11244 |
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author | Sharma, Vishal Pope, Brandon J. Santiago, Nathan V. Boland, Molly T. Sun, Dongmei Reynolds, Richard J. Szalai, Alexander J. Bridges, S. Louis Raman, Chander |
author_facet | Sharma, Vishal Pope, Brandon J. Santiago, Nathan V. Boland, Molly T. Sun, Dongmei Reynolds, Richard J. Szalai, Alexander J. Bridges, S. Louis Raman, Chander |
author_sort | Sharma, Vishal |
collection | PubMed |
description | OBJECTIVE: We investigated whether a previously reported association of IFNGR expression with rheumatoid arthritis (RA) and its radiographic severity reflects differences in proximal interferon‐γ (IFN‐γ) signaling in T cells from patients with RA compared with healthy controls (HC). METHODS: Using phosphoflow cytometry, we compared IFN‐γ–stimulated signal transducer and activator of transcription 1 (STAT1) activation in CD4(+) and CD8(+) T‐cell populations from patients with RA and HC. RESULTS: Compared with controls, patients with RA had a higher proportion of CD4(+) T cells, associated with expansion of the CD4(+) effector memory subset. Several CD4(+) T‐cell types exhibited reduced IFN‐γ–induced phosphoSTAT1(Y701) (pSTAT1(Y701)) in patients with RA compared with HC. Engaging the T‐cell receptor (TCR) complex on CD4(+) T cells during IFN‐γ stimulation abrogated the reduction in STAT1 activation in patients with RA but had no effect in HC. The phosphorylation of STAT1(S727) was similar in CD4(+) T cells from patients with RA and HC. In contrast to CD4(+) T cells, IFN‐γ–induced pSTAT1(Y701) levels in CD8(+) T cells were equivalent or higher in patients with RA compared with HC. Total STAT1 levels (phosphorylated + unphosphorylated) were lower in CD4(+) and CD8(+) T cells from patients with RA compared with HC. CONCLUSION: We report diminished IFN‐γ–induced pSTAT1(Y701) levels in CD4(+) T cells in patients with RA, which were restored by TCR engagement. There were lower levels of total STAT1 in patients with RA compared with HC, but this likely does not explain diminished IFN‐γ–induced pSTAT1(Y701) levels in CD4(+) T cells because activation in CD8(+) T cells was higher or equivalent to that seen in HC. The enhanced IFNGR expression in patients with RA reported previously may reflect a compensatory mechanism to overcome deficiency in IFN‐γ responsiveness. |
format | Online Article Text |
id | pubmed-8063148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80631482021-04-23 Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells Sharma, Vishal Pope, Brandon J. Santiago, Nathan V. Boland, Molly T. Sun, Dongmei Reynolds, Richard J. Szalai, Alexander J. Bridges, S. Louis Raman, Chander ACR Open Rheumatol Brief Report OBJECTIVE: We investigated whether a previously reported association of IFNGR expression with rheumatoid arthritis (RA) and its radiographic severity reflects differences in proximal interferon‐γ (IFN‐γ) signaling in T cells from patients with RA compared with healthy controls (HC). METHODS: Using phosphoflow cytometry, we compared IFN‐γ–stimulated signal transducer and activator of transcription 1 (STAT1) activation in CD4(+) and CD8(+) T‐cell populations from patients with RA and HC. RESULTS: Compared with controls, patients with RA had a higher proportion of CD4(+) T cells, associated with expansion of the CD4(+) effector memory subset. Several CD4(+) T‐cell types exhibited reduced IFN‐γ–induced phosphoSTAT1(Y701) (pSTAT1(Y701)) in patients with RA compared with HC. Engaging the T‐cell receptor (TCR) complex on CD4(+) T cells during IFN‐γ stimulation abrogated the reduction in STAT1 activation in patients with RA but had no effect in HC. The phosphorylation of STAT1(S727) was similar in CD4(+) T cells from patients with RA and HC. In contrast to CD4(+) T cells, IFN‐γ–induced pSTAT1(Y701) levels in CD8(+) T cells were equivalent or higher in patients with RA compared with HC. Total STAT1 levels (phosphorylated + unphosphorylated) were lower in CD4(+) and CD8(+) T cells from patients with RA compared with HC. CONCLUSION: We report diminished IFN‐γ–induced pSTAT1(Y701) levels in CD4(+) T cells in patients with RA, which were restored by TCR engagement. There were lower levels of total STAT1 in patients with RA compared with HC, but this likely does not explain diminished IFN‐γ–induced pSTAT1(Y701) levels in CD4(+) T cells because activation in CD8(+) T cells was higher or equivalent to that seen in HC. The enhanced IFNGR expression in patients with RA reported previously may reflect a compensatory mechanism to overcome deficiency in IFN‐γ responsiveness. John Wiley and Sons Inc. 2021-03-28 /pmc/articles/PMC8063148/ /pubmed/33779079 http://dx.doi.org/10.1002/acr2.11244 Text en © 2021 The Authors. ACR Open Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Brief Report Sharma, Vishal Pope, Brandon J. Santiago, Nathan V. Boland, Molly T. Sun, Dongmei Reynolds, Richard J. Szalai, Alexander J. Bridges, S. Louis Raman, Chander Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title | Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title_full | Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title_fullStr | Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title_full_unstemmed | Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title_short | Decreased Levels of STAT1 and Interferon‐γ–Induced STAT1 Phosphorylation in Rheumatoid Arthritis CD4 and CD8 T Cells |
title_sort | decreased levels of stat1 and interferon‐γ–induced stat1 phosphorylation in rheumatoid arthritis cd4 and cd8 t cells |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063148/ https://www.ncbi.nlm.nih.gov/pubmed/33779079 http://dx.doi.org/10.1002/acr2.11244 |
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