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Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks

Diacylglycerol acyl-transferase (DGAT) and cysteine oleosin (CO) expression confers a novel carbon sink (of encapsulated lipid droplets) in leaves of Lolium perenne and has been shown to increase photosynthesis and biomass. However, the physiological mechanism by which DGAT + CO increases photosynth...

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Autores principales: Cooney, Luke J., Beechey-Gradwell, Zac, Winichayakul, Somrutai, Richardson, Kim A., Crowther, Tracey, Anderson, Philip, Scott, Richard W., Bryan, Gregory, Roberts, Nicholas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063613/
https://www.ncbi.nlm.nih.gov/pubmed/33897730
http://dx.doi.org/10.3389/fpls.2021.641822
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author Cooney, Luke J.
Beechey-Gradwell, Zac
Winichayakul, Somrutai
Richardson, Kim A.
Crowther, Tracey
Anderson, Philip
Scott, Richard W.
Bryan, Gregory
Roberts, Nicholas J.
author_facet Cooney, Luke J.
Beechey-Gradwell, Zac
Winichayakul, Somrutai
Richardson, Kim A.
Crowther, Tracey
Anderson, Philip
Scott, Richard W.
Bryan, Gregory
Roberts, Nicholas J.
author_sort Cooney, Luke J.
collection PubMed
description Diacylglycerol acyl-transferase (DGAT) and cysteine oleosin (CO) expression confers a novel carbon sink (of encapsulated lipid droplets) in leaves of Lolium perenne and has been shown to increase photosynthesis and biomass. However, the physiological mechanism by which DGAT + CO increases photosynthesis remains unresolved. To evaluate the relationship between sink strength and photosynthesis, we examined fatty acids (FA), water-soluble carbohydrates (WSC), gas exchange parameters and leaf nitrogen for multiple DGAT + CO lines varying in transgene accumulation. To identify the physiological traits which deliver increased photosynthesis, we assessed two important determinants of photosynthetic efficiency, CO(2) conductance from atmosphere to chloroplast, and nitrogen partitioning between different photosynthetic and non-photosynthetic pools. We found that DGAT + CO accumulation increased FA at the expense of WSC in leaves of L. perenne and for those lines with a significant reduction in WSC, we also observed an increase in photosynthesis and photosynthetic nitrogen use efficiency. DGAT + CO L. perenne displayed no change in rubisco content or V(cmax) but did exhibit a significant increase in specific leaf area (SLA), stomatal and mesophyll conductance, and leaf nitrogen allocated to photosynthetic electron transport. Collectively, we showed that increased carbon demand via DGAT+CO lipid sink accumulation can induce leaf-level changes in L. perenne which deliver increased rates of photosynthesis and growth. Carbon sinks engineered within photosynthetic cells provide a promising new strategy for increasing photosynthesis and crop productivity.
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spelling pubmed-80636132021-04-24 Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks Cooney, Luke J. Beechey-Gradwell, Zac Winichayakul, Somrutai Richardson, Kim A. Crowther, Tracey Anderson, Philip Scott, Richard W. Bryan, Gregory Roberts, Nicholas J. Front Plant Sci Plant Science Diacylglycerol acyl-transferase (DGAT) and cysteine oleosin (CO) expression confers a novel carbon sink (of encapsulated lipid droplets) in leaves of Lolium perenne and has been shown to increase photosynthesis and biomass. However, the physiological mechanism by which DGAT + CO increases photosynthesis remains unresolved. To evaluate the relationship between sink strength and photosynthesis, we examined fatty acids (FA), water-soluble carbohydrates (WSC), gas exchange parameters and leaf nitrogen for multiple DGAT + CO lines varying in transgene accumulation. To identify the physiological traits which deliver increased photosynthesis, we assessed two important determinants of photosynthetic efficiency, CO(2) conductance from atmosphere to chloroplast, and nitrogen partitioning between different photosynthetic and non-photosynthetic pools. We found that DGAT + CO accumulation increased FA at the expense of WSC in leaves of L. perenne and for those lines with a significant reduction in WSC, we also observed an increase in photosynthesis and photosynthetic nitrogen use efficiency. DGAT + CO L. perenne displayed no change in rubisco content or V(cmax) but did exhibit a significant increase in specific leaf area (SLA), stomatal and mesophyll conductance, and leaf nitrogen allocated to photosynthetic electron transport. Collectively, we showed that increased carbon demand via DGAT+CO lipid sink accumulation can induce leaf-level changes in L. perenne which deliver increased rates of photosynthesis and growth. Carbon sinks engineered within photosynthetic cells provide a promising new strategy for increasing photosynthesis and crop productivity. Frontiers Media S.A. 2021-03-09 /pmc/articles/PMC8063613/ /pubmed/33897730 http://dx.doi.org/10.3389/fpls.2021.641822 Text en Copyright © 2021 Cooney, Beechey-Gradwell, Winichayakul, Richardson, Crowther, Anderson, Scott, Bryan and Roberts. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cooney, Luke J.
Beechey-Gradwell, Zac
Winichayakul, Somrutai
Richardson, Kim A.
Crowther, Tracey
Anderson, Philip
Scott, Richard W.
Bryan, Gregory
Roberts, Nicholas J.
Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title_full Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title_fullStr Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title_full_unstemmed Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title_short Changes in Leaf-Level Nitrogen Partitioning and Mesophyll Conductance Deliver Increased Photosynthesis for Lolium perenne Leaves Engineered to Accumulate Lipid Carbon Sinks
title_sort changes in leaf-level nitrogen partitioning and mesophyll conductance deliver increased photosynthesis for lolium perenne leaves engineered to accumulate lipid carbon sinks
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063613/
https://www.ncbi.nlm.nih.gov/pubmed/33897730
http://dx.doi.org/10.3389/fpls.2021.641822
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