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AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria

Antimicrobial peptides (AMPs), which are evolutionarily conserved components of the innate immune response, contribute to the first line of defense against microbes in the skin and at mucosal surfaces. Here, we report the identification of a human peptide, encoded by the chromosome 5 open reading fr...

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Autores principales: Zhong, Kunhong, Wang, Yuelong, Wang, Zeng, Zhang, Zongliang, Zhao, Shasha, Li, Hexian, Huang, Jianhan, Guo, Wenhao, Zheng, Xi, Guo, Gang, Zhou, Liangxue, Yang, Hui, Tong, Aiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063792/
https://www.ncbi.nlm.nih.gov/pubmed/33804835
http://dx.doi.org/10.3390/biom11040485
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author Zhong, Kunhong
Wang, Yuelong
Wang, Zeng
Zhang, Zongliang
Zhao, Shasha
Li, Hexian
Huang, Jianhan
Guo, Wenhao
Zheng, Xi
Guo, Gang
Zhou, Liangxue
Yang, Hui
Tong, Aiping
author_facet Zhong, Kunhong
Wang, Yuelong
Wang, Zeng
Zhang, Zongliang
Zhao, Shasha
Li, Hexian
Huang, Jianhan
Guo, Wenhao
Zheng, Xi
Guo, Gang
Zhou, Liangxue
Yang, Hui
Tong, Aiping
author_sort Zhong, Kunhong
collection PubMed
description Antimicrobial peptides (AMPs), which are evolutionarily conserved components of the innate immune response, contribute to the first line of defense against microbes in the skin and at mucosal surfaces. Here, we report the identification of a human peptide, encoded by the chromosome 5 open reading frame 46 (C5orf46) gene, as a type of AMP, which we termed antimicrobial peptide with 64 amino acid residues (AP-64). AP-64 is an anionic amphiphilic peptide lacking cysteines (MW = 7.2, PI = 4.54). AP-64 exhibited significant antibacterial activity against Gram-negative bacteria, including Escherichia coli DH5α, Escherichia coli O157:H7, Vibrio cholerae, and Pseudomonas aeruginosa. Moreover, AP-64 was efficient in combating Escherichia coli O157:H7 infections in a mouse model and exhibited cytotoxic effects against human T-cell lymphoma Jurkat and B-cell lymphoma Raji cells. We also observed that Gm94, encoded by mouse C5orf46 homologous gene, closely resembles AP-64 in its antibacterial properties. Compared with other human AMPs, AP-64 has distinct characteristics, including a longer sequence length, absence of cysteine residues, a highly anionic character, and cell toxicity. Together, this study identified that AP-64 is an AMP worthy of further investigation.
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spelling pubmed-80637922021-04-24 AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria Zhong, Kunhong Wang, Yuelong Wang, Zeng Zhang, Zongliang Zhao, Shasha Li, Hexian Huang, Jianhan Guo, Wenhao Zheng, Xi Guo, Gang Zhou, Liangxue Yang, Hui Tong, Aiping Biomolecules Article Antimicrobial peptides (AMPs), which are evolutionarily conserved components of the innate immune response, contribute to the first line of defense against microbes in the skin and at mucosal surfaces. Here, we report the identification of a human peptide, encoded by the chromosome 5 open reading frame 46 (C5orf46) gene, as a type of AMP, which we termed antimicrobial peptide with 64 amino acid residues (AP-64). AP-64 is an anionic amphiphilic peptide lacking cysteines (MW = 7.2, PI = 4.54). AP-64 exhibited significant antibacterial activity against Gram-negative bacteria, including Escherichia coli DH5α, Escherichia coli O157:H7, Vibrio cholerae, and Pseudomonas aeruginosa. Moreover, AP-64 was efficient in combating Escherichia coli O157:H7 infections in a mouse model and exhibited cytotoxic effects against human T-cell lymphoma Jurkat and B-cell lymphoma Raji cells. We also observed that Gm94, encoded by mouse C5orf46 homologous gene, closely resembles AP-64 in its antibacterial properties. Compared with other human AMPs, AP-64 has distinct characteristics, including a longer sequence length, absence of cysteine residues, a highly anionic character, and cell toxicity. Together, this study identified that AP-64 is an AMP worthy of further investigation. MDPI 2021-03-24 /pmc/articles/PMC8063792/ /pubmed/33804835 http://dx.doi.org/10.3390/biom11040485 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Zhong, Kunhong
Wang, Yuelong
Wang, Zeng
Zhang, Zongliang
Zhao, Shasha
Li, Hexian
Huang, Jianhan
Guo, Wenhao
Zheng, Xi
Guo, Gang
Zhou, Liangxue
Yang, Hui
Tong, Aiping
AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title_full AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title_fullStr AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title_full_unstemmed AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title_short AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria
title_sort ap-64, encoded by c5orf46, exhibits antimicrobial activity against gram-negative bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063792/
https://www.ncbi.nlm.nih.gov/pubmed/33804835
http://dx.doi.org/10.3390/biom11040485
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