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Effect of Long-Term 3D Spheroid Culture on WJ-MSC

The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture con...

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Detalles Bibliográficos
Autores principales: Kaminska, Agnieszka, Wedzinska, Aleksandra, Kot, Marta, Sarnowska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063822/
https://www.ncbi.nlm.nih.gov/pubmed/33804895
http://dx.doi.org/10.3390/cells10040719
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author Kaminska, Agnieszka
Wedzinska, Aleksandra
Kot, Marta
Sarnowska, Anna
author_facet Kaminska, Agnieszka
Wedzinska, Aleksandra
Kot, Marta
Sarnowska, Anna
author_sort Kaminska, Agnieszka
collection PubMed
description The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in 3D neurosphere induction medium for about 20 days in vitro. Then, cells were transported to 2D conditions and confront to the initial population and population constantly cultured in 2D. 3D spheroids culture of WJ-MSC resulted in increased senescence, decreased stemness and proliferation. However long-termed 3D spheroid culture allowed for selection of cells exhibiting increased expression of early neural and SSEA4 markers what might indicate the survival of cell subpopulation with unique features.
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spelling pubmed-80638222021-04-24 Effect of Long-Term 3D Spheroid Culture on WJ-MSC Kaminska, Agnieszka Wedzinska, Aleksandra Kot, Marta Sarnowska, Anna Cells Article The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in 3D neurosphere induction medium for about 20 days in vitro. Then, cells were transported to 2D conditions and confront to the initial population and population constantly cultured in 2D. 3D spheroids culture of WJ-MSC resulted in increased senescence, decreased stemness and proliferation. However long-termed 3D spheroid culture allowed for selection of cells exhibiting increased expression of early neural and SSEA4 markers what might indicate the survival of cell subpopulation with unique features. MDPI 2021-03-24 /pmc/articles/PMC8063822/ /pubmed/33804895 http://dx.doi.org/10.3390/cells10040719 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Kaminska, Agnieszka
Wedzinska, Aleksandra
Kot, Marta
Sarnowska, Anna
Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title_full Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title_fullStr Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title_full_unstemmed Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title_short Effect of Long-Term 3D Spheroid Culture on WJ-MSC
title_sort effect of long-term 3d spheroid culture on wj-msc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063822/
https://www.ncbi.nlm.nih.gov/pubmed/33804895
http://dx.doi.org/10.3390/cells10040719
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