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Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe

Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infe...

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Autores principales: Bali, Krisztina, Bálint, Ádám, Farsang, Attila, Marton, Szilvia, Nagy, Borbála, Kaszab, Eszter, Belák, Sándor, Palya, Vilmos, Bányai, Krisztián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063831/
https://www.ncbi.nlm.nih.gov/pubmed/33804824
http://dx.doi.org/10.3390/v13040535
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author Bali, Krisztina
Bálint, Ádám
Farsang, Attila
Marton, Szilvia
Nagy, Borbála
Kaszab, Eszter
Belák, Sándor
Palya, Vilmos
Bányai, Krisztián
author_facet Bali, Krisztina
Bálint, Ádám
Farsang, Attila
Marton, Szilvia
Nagy, Borbála
Kaszab, Eszter
Belák, Sándor
Palya, Vilmos
Bányai, Krisztián
author_sort Bali, Krisztina
collection PubMed
description Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infectious bronchitis virus (IBV) strains using phylogenetic and recombination analysis. We sequenced the genome of 20 randomly selected strains from seven European countries. After sequencing, we created a genome sequence data set that contained 36 European origin field isolates and 33 vaccine strains. When analyzing these 69 IBV genome sequences, we identified 215 recombination events highlighting that some strains had multiple recombination breaking points. Recombination hot spots were identified mostly in the regions coding for non-structural proteins, and multiple recombination hot spots were identified in the nsp2, nsp3, nsp8, and nsp12 coding regions. Recombination occurred among different IBV genotypes and involved both field and vaccine IBV strains. Ninety percent of field strains and nearly half of vaccine strains showed evidence of recombination. Despite the low number and the scattered geographical and temporal origin of whole-genome sequence data collected from European Gammacoronaviruses, this study underlines the importance of recombination as a major evolutionary mechanism of IBVs.
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spelling pubmed-80638312021-04-24 Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe Bali, Krisztina Bálint, Ádám Farsang, Attila Marton, Szilvia Nagy, Borbála Kaszab, Eszter Belák, Sándor Palya, Vilmos Bányai, Krisztián Viruses Article Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infectious bronchitis virus (IBV) strains using phylogenetic and recombination analysis. We sequenced the genome of 20 randomly selected strains from seven European countries. After sequencing, we created a genome sequence data set that contained 36 European origin field isolates and 33 vaccine strains. When analyzing these 69 IBV genome sequences, we identified 215 recombination events highlighting that some strains had multiple recombination breaking points. Recombination hot spots were identified mostly in the regions coding for non-structural proteins, and multiple recombination hot spots were identified in the nsp2, nsp3, nsp8, and nsp12 coding regions. Recombination occurred among different IBV genotypes and involved both field and vaccine IBV strains. Ninety percent of field strains and nearly half of vaccine strains showed evidence of recombination. Despite the low number and the scattered geographical and temporal origin of whole-genome sequence data collected from European Gammacoronaviruses, this study underlines the importance of recombination as a major evolutionary mechanism of IBVs. MDPI 2021-03-24 /pmc/articles/PMC8063831/ /pubmed/33804824 http://dx.doi.org/10.3390/v13040535 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Bali, Krisztina
Bálint, Ádám
Farsang, Attila
Marton, Szilvia
Nagy, Borbála
Kaszab, Eszter
Belák, Sándor
Palya, Vilmos
Bányai, Krisztián
Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title_full Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title_fullStr Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title_full_unstemmed Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title_short Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe
title_sort recombination events shape the genomic evolution of infectious bronchitis virus in europe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063831/
https://www.ncbi.nlm.nih.gov/pubmed/33804824
http://dx.doi.org/10.3390/v13040535
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