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Identification and comparison of circular RNAs in preeclampsia

BACKGROUND: Preeclampsia (PE) is a pregnancy-specific syndrome, belongs to the gestational hypertension diseases category and is considered among the causes of maternal and perinatal mortality and morbidity. However, the pathogenesis of PE is still vague. METHODS: In the present study, the circular...

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Autores principales: Ping, Zepeng, Ai, Ling, Shen, Huaxiang, Zhang, Xing, Jiang, Huling, Song, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063878/
https://www.ncbi.nlm.nih.gov/pubmed/33976984
http://dx.doi.org/10.7717/peerj.11299
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author Ping, Zepeng
Ai, Ling
Shen, Huaxiang
Zhang, Xing
Jiang, Huling
Song, Ye
author_facet Ping, Zepeng
Ai, Ling
Shen, Huaxiang
Zhang, Xing
Jiang, Huling
Song, Ye
author_sort Ping, Zepeng
collection PubMed
description BACKGROUND: Preeclampsia (PE) is a pregnancy-specific syndrome, belongs to the gestational hypertension diseases category and is considered among the causes of maternal and perinatal mortality and morbidity. However, the pathogenesis of PE is still vague. METHODS: In the present study, the circular RNA (circRNA) expression patterns of normal pregnant women and PE patients were investigated using whole RNA sequencing. RESULTS: A total of 151 differential expressed circRNAs were identified including 121 upregulated and 30 downregulated ones. Functional and pathway enrichment analysis was conducted on the differentially expressed circRNAs using Gene Ontology and KEGG databases. The results of this analysis indicated that several crucial biological processes and pathways were enriched in PE patients. circRNA–microRNA (miRNA) interaction analysis indicated that the reported differentially expresse circRNAs may be associated with some regulatory functions through miRNAs in PE patients. Two ceRNAs networks were constructed according to the targeting relationship between circRNAs/miRNAs and miRNAs/mRNAs. One sub-network contained one upregulated circRNA, four downregulated miRNAs and five upregulated mRNAs, and another sub-network contained 10 downregulated circRNAs, 21 upregulated miRNAs and 15 downregulated mRNAs. CONCLUSION: CircRNA expression patterns have been investigated and this analysis revealed their potential regulatory mechanisms in PE patients. We constructed the ceRNAs (competing endogenous RNA) to reveal the potential molecular roles of dysregulated circRNAs in the PE patients using RNA sequencing data. circRNA_13301 was the only one upregulated circRNA in ceRNA being targeted by four miRNAs.
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spelling pubmed-80638782021-05-10 Identification and comparison of circular RNAs in preeclampsia Ping, Zepeng Ai, Ling Shen, Huaxiang Zhang, Xing Jiang, Huling Song, Ye PeerJ Bioinformatics BACKGROUND: Preeclampsia (PE) is a pregnancy-specific syndrome, belongs to the gestational hypertension diseases category and is considered among the causes of maternal and perinatal mortality and morbidity. However, the pathogenesis of PE is still vague. METHODS: In the present study, the circular RNA (circRNA) expression patterns of normal pregnant women and PE patients were investigated using whole RNA sequencing. RESULTS: A total of 151 differential expressed circRNAs were identified including 121 upregulated and 30 downregulated ones. Functional and pathway enrichment analysis was conducted on the differentially expressed circRNAs using Gene Ontology and KEGG databases. The results of this analysis indicated that several crucial biological processes and pathways were enriched in PE patients. circRNA–microRNA (miRNA) interaction analysis indicated that the reported differentially expresse circRNAs may be associated with some regulatory functions through miRNAs in PE patients. Two ceRNAs networks were constructed according to the targeting relationship between circRNAs/miRNAs and miRNAs/mRNAs. One sub-network contained one upregulated circRNA, four downregulated miRNAs and five upregulated mRNAs, and another sub-network contained 10 downregulated circRNAs, 21 upregulated miRNAs and 15 downregulated mRNAs. CONCLUSION: CircRNA expression patterns have been investigated and this analysis revealed their potential regulatory mechanisms in PE patients. We constructed the ceRNAs (competing endogenous RNA) to reveal the potential molecular roles of dysregulated circRNAs in the PE patients using RNA sequencing data. circRNA_13301 was the only one upregulated circRNA in ceRNA being targeted by four miRNAs. PeerJ Inc. 2021-04-20 /pmc/articles/PMC8063878/ /pubmed/33976984 http://dx.doi.org/10.7717/peerj.11299 Text en ©2021 Ping et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Ping, Zepeng
Ai, Ling
Shen, Huaxiang
Zhang, Xing
Jiang, Huling
Song, Ye
Identification and comparison of circular RNAs in preeclampsia
title Identification and comparison of circular RNAs in preeclampsia
title_full Identification and comparison of circular RNAs in preeclampsia
title_fullStr Identification and comparison of circular RNAs in preeclampsia
title_full_unstemmed Identification and comparison of circular RNAs in preeclampsia
title_short Identification and comparison of circular RNAs in preeclampsia
title_sort identification and comparison of circular rnas in preeclampsia
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063878/
https://www.ncbi.nlm.nih.gov/pubmed/33976984
http://dx.doi.org/10.7717/peerj.11299
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