Cargando…

A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity

There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with f...

Descripción completa

Detalles Bibliográficos
Autores principales: Hwang, Ji-Hyeon, Lee, Gyeongmin, Kim, Aro, Park, Jong-Hyeon, Lee, Min Ja, Kim, Byounghan, Kim, Su-Mi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063925/
https://www.ncbi.nlm.nih.gov/pubmed/33805012
http://dx.doi.org/10.3390/vaccines9040308
_version_ 1783682037357477888
author Hwang, Ji-Hyeon
Lee, Gyeongmin
Kim, Aro
Park, Jong-Hyeon
Lee, Min Ja
Kim, Byounghan
Kim, Su-Mi
author_facet Hwang, Ji-Hyeon
Lee, Gyeongmin
Kim, Aro
Park, Jong-Hyeon
Lee, Min Ja
Kim, Byounghan
Kim, Su-Mi
author_sort Hwang, Ji-Hyeon
collection PubMed
description There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with field isolates. Therefore, the topotype/lineage vaccine strain circulating in many countries and a highly immunogenic strain might be advantageous to control serotype A FMDV. We developed a new vaccine strain, A/SKR/Yeoncheon/2017 (A-1), which belongs to the A/ASIA/Sea-97 lineage that frequently occurs in Asian countries. Using virus plaque purification, we selected a vaccine virus with high antigen productivity and the lowest numbers of P1 mutations among cell-adapted virus populations. The A/SKR/Yeoncheon/2017 (A-1) vaccine strain has a single amino acid mutation, VP2 E82K, in the P1 region, and it is perfectly adapted to suspension culture. The A/SKR/Yeoncheon/2017 (A-1) experimental vaccine conferred high immunogenicity in pigs. The vaccine strain was serologically matched with various field isolates in two-dimensional virus neutralization tests using bovine serum. Vaccinated mice were protected against an A/MAY/97 virus that was serologically mismatched with the vaccine strain. Thus, A/SKR/Yeoncheon/2017 (A-1) might be a promising vaccine candidate for protection against the emerging FMDV serotype A in Asia.
format Online
Article
Text
id pubmed-8063925
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80639252021-04-24 A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity Hwang, Ji-Hyeon Lee, Gyeongmin Kim, Aro Park, Jong-Hyeon Lee, Min Ja Kim, Byounghan Kim, Su-Mi Vaccines (Basel) Article There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with field isolates. Therefore, the topotype/lineage vaccine strain circulating in many countries and a highly immunogenic strain might be advantageous to control serotype A FMDV. We developed a new vaccine strain, A/SKR/Yeoncheon/2017 (A-1), which belongs to the A/ASIA/Sea-97 lineage that frequently occurs in Asian countries. Using virus plaque purification, we selected a vaccine virus with high antigen productivity and the lowest numbers of P1 mutations among cell-adapted virus populations. The A/SKR/Yeoncheon/2017 (A-1) vaccine strain has a single amino acid mutation, VP2 E82K, in the P1 region, and it is perfectly adapted to suspension culture. The A/SKR/Yeoncheon/2017 (A-1) experimental vaccine conferred high immunogenicity in pigs. The vaccine strain was serologically matched with various field isolates in two-dimensional virus neutralization tests using bovine serum. Vaccinated mice were protected against an A/MAY/97 virus that was serologically mismatched with the vaccine strain. Thus, A/SKR/Yeoncheon/2017 (A-1) might be a promising vaccine candidate for protection against the emerging FMDV serotype A in Asia. MDPI 2021-03-24 /pmc/articles/PMC8063925/ /pubmed/33805012 http://dx.doi.org/10.3390/vaccines9040308 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Hwang, Ji-Hyeon
Lee, Gyeongmin
Kim, Aro
Park, Jong-Hyeon
Lee, Min Ja
Kim, Byounghan
Kim, Su-Mi
A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title_full A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title_fullStr A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title_full_unstemmed A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title_short A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
title_sort vaccine strain of the a/asia/sea-97 lineage of foot-and-mouth disease virus with a single amino acid substitution in the p1 region that is adapted to suspension culture provides high immunogenicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063925/
https://www.ncbi.nlm.nih.gov/pubmed/33805012
http://dx.doi.org/10.3390/vaccines9040308
work_keys_str_mv AT hwangjihyeon avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT leegyeongmin avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimaro avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT parkjonghyeon avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT leeminja avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimbyounghan avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimsumi avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT hwangjihyeon vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT leegyeongmin vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimaro vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT parkjonghyeon vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT leeminja vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimbyounghan vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity
AT kimsumi vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity