Cargando…
A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity
There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with f...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063925/ https://www.ncbi.nlm.nih.gov/pubmed/33805012 http://dx.doi.org/10.3390/vaccines9040308 |
_version_ | 1783682037357477888 |
---|---|
author | Hwang, Ji-Hyeon Lee, Gyeongmin Kim, Aro Park, Jong-Hyeon Lee, Min Ja Kim, Byounghan Kim, Su-Mi |
author_facet | Hwang, Ji-Hyeon Lee, Gyeongmin Kim, Aro Park, Jong-Hyeon Lee, Min Ja Kim, Byounghan Kim, Su-Mi |
author_sort | Hwang, Ji-Hyeon |
collection | PubMed |
description | There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with field isolates. Therefore, the topotype/lineage vaccine strain circulating in many countries and a highly immunogenic strain might be advantageous to control serotype A FMDV. We developed a new vaccine strain, A/SKR/Yeoncheon/2017 (A-1), which belongs to the A/ASIA/Sea-97 lineage that frequently occurs in Asian countries. Using virus plaque purification, we selected a vaccine virus with high antigen productivity and the lowest numbers of P1 mutations among cell-adapted virus populations. The A/SKR/Yeoncheon/2017 (A-1) vaccine strain has a single amino acid mutation, VP2 E82K, in the P1 region, and it is perfectly adapted to suspension culture. The A/SKR/Yeoncheon/2017 (A-1) experimental vaccine conferred high immunogenicity in pigs. The vaccine strain was serologically matched with various field isolates in two-dimensional virus neutralization tests using bovine serum. Vaccinated mice were protected against an A/MAY/97 virus that was serologically mismatched with the vaccine strain. Thus, A/SKR/Yeoncheon/2017 (A-1) might be a promising vaccine candidate for protection against the emerging FMDV serotype A in Asia. |
format | Online Article Text |
id | pubmed-8063925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80639252021-04-24 A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity Hwang, Ji-Hyeon Lee, Gyeongmin Kim, Aro Park, Jong-Hyeon Lee, Min Ja Kim, Byounghan Kim, Su-Mi Vaccines (Basel) Article There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with field isolates. Therefore, the topotype/lineage vaccine strain circulating in many countries and a highly immunogenic strain might be advantageous to control serotype A FMDV. We developed a new vaccine strain, A/SKR/Yeoncheon/2017 (A-1), which belongs to the A/ASIA/Sea-97 lineage that frequently occurs in Asian countries. Using virus plaque purification, we selected a vaccine virus with high antigen productivity and the lowest numbers of P1 mutations among cell-adapted virus populations. The A/SKR/Yeoncheon/2017 (A-1) vaccine strain has a single amino acid mutation, VP2 E82K, in the P1 region, and it is perfectly adapted to suspension culture. The A/SKR/Yeoncheon/2017 (A-1) experimental vaccine conferred high immunogenicity in pigs. The vaccine strain was serologically matched with various field isolates in two-dimensional virus neutralization tests using bovine serum. Vaccinated mice were protected against an A/MAY/97 virus that was serologically mismatched with the vaccine strain. Thus, A/SKR/Yeoncheon/2017 (A-1) might be a promising vaccine candidate for protection against the emerging FMDV serotype A in Asia. MDPI 2021-03-24 /pmc/articles/PMC8063925/ /pubmed/33805012 http://dx.doi.org/10.3390/vaccines9040308 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Hwang, Ji-Hyeon Lee, Gyeongmin Kim, Aro Park, Jong-Hyeon Lee, Min Ja Kim, Byounghan Kim, Su-Mi A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title | A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title_full | A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title_fullStr | A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title_full_unstemmed | A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title_short | A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity |
title_sort | vaccine strain of the a/asia/sea-97 lineage of foot-and-mouth disease virus with a single amino acid substitution in the p1 region that is adapted to suspension culture provides high immunogenicity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063925/ https://www.ncbi.nlm.nih.gov/pubmed/33805012 http://dx.doi.org/10.3390/vaccines9040308 |
work_keys_str_mv | AT hwangjihyeon avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT leegyeongmin avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimaro avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT parkjonghyeon avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT leeminja avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimbyounghan avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimsumi avaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT hwangjihyeon vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT leegyeongmin vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimaro vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT parkjonghyeon vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT leeminja vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimbyounghan vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity AT kimsumi vaccinestrainoftheaasiasea97lineageoffootandmouthdiseaseviruswithasingleaminoacidsubstitutioninthep1regionthatisadaptedtosuspensioncultureprovideshighimmunogenicity |