Cargando…
Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates
The unprecedented and rapid spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) has motivated the need for a rapidly producible and scalable vaccine. Here, we developed a synthetic soluble SARS-CoV-2 spike (S) DNA-based vaccine candidate, GX-19. In mice, immunization with GX-19 el...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063950/ https://www.ncbi.nlm.nih.gov/pubmed/33804981 http://dx.doi.org/10.3390/vaccines9040307 |
_version_ | 1783682043131985920 |
---|---|
author | Seo, Yong Bok Suh, You Suk Ryu, Ji In Jang, Hwanhee Oh, Hanseul Koo, Bon-Sang Seo, Sang-Hwan Hong, Jung Joo Song, Manki Kim, Sung-Joo Sung, Young Chul |
author_facet | Seo, Yong Bok Suh, You Suk Ryu, Ji In Jang, Hwanhee Oh, Hanseul Koo, Bon-Sang Seo, Sang-Hwan Hong, Jung Joo Song, Manki Kim, Sung-Joo Sung, Young Chul |
author_sort | Seo, Yong Bok |
collection | PubMed |
description | The unprecedented and rapid spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) has motivated the need for a rapidly producible and scalable vaccine. Here, we developed a synthetic soluble SARS-CoV-2 spike (S) DNA-based vaccine candidate, GX-19. In mice, immunization with GX-19 elicited not only S-specific systemic and pulmonary antibody responses but also Th1-biased T cell responses in a dose-dependent manner. GX-19-vaccinated nonhuman primates seroconverted rapidly and exhibited a detectable neutralizing antibody response as well as multifunctional CD4+ and CD8+ T cell responses. Notably, when the immunized nonhuman primates were challenged at 10 weeks after the last vaccination with GX-19, they had reduced viral loads in contrast to non-vaccinated primates as a control. These findings indicate that GX-19 vaccination provides a durable protective immune response and also support further development of GX-19 as a vaccine candidate for SARS-CoV-2. |
format | Online Article Text |
id | pubmed-8063950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80639502021-04-24 Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates Seo, Yong Bok Suh, You Suk Ryu, Ji In Jang, Hwanhee Oh, Hanseul Koo, Bon-Sang Seo, Sang-Hwan Hong, Jung Joo Song, Manki Kim, Sung-Joo Sung, Young Chul Vaccines (Basel) Article The unprecedented and rapid spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) has motivated the need for a rapidly producible and scalable vaccine. Here, we developed a synthetic soluble SARS-CoV-2 spike (S) DNA-based vaccine candidate, GX-19. In mice, immunization with GX-19 elicited not only S-specific systemic and pulmonary antibody responses but also Th1-biased T cell responses in a dose-dependent manner. GX-19-vaccinated nonhuman primates seroconverted rapidly and exhibited a detectable neutralizing antibody response as well as multifunctional CD4+ and CD8+ T cell responses. Notably, when the immunized nonhuman primates were challenged at 10 weeks after the last vaccination with GX-19, they had reduced viral loads in contrast to non-vaccinated primates as a control. These findings indicate that GX-19 vaccination provides a durable protective immune response and also support further development of GX-19 as a vaccine candidate for SARS-CoV-2. MDPI 2021-03-24 /pmc/articles/PMC8063950/ /pubmed/33804981 http://dx.doi.org/10.3390/vaccines9040307 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Seo, Yong Bok Suh, You Suk Ryu, Ji In Jang, Hwanhee Oh, Hanseul Koo, Bon-Sang Seo, Sang-Hwan Hong, Jung Joo Song, Manki Kim, Sung-Joo Sung, Young Chul Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title | Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title_full | Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title_fullStr | Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title_full_unstemmed | Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title_short | Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman Primates |
title_sort | soluble spike dna vaccine provides long-term protective immunity against sars-cov-2 in mice and nonhuman primates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063950/ https://www.ncbi.nlm.nih.gov/pubmed/33804981 http://dx.doi.org/10.3390/vaccines9040307 |
work_keys_str_mv | AT seoyongbok solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT suhyousuk solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT ryujiin solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT janghwanhee solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT ohhanseul solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT koobonsang solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT seosanghwan solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT hongjungjoo solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT songmanki solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT kimsungjoo solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates AT sungyoungchul solublespikednavaccineprovideslongtermprotectiveimmunityagainstsarscov2inmiceandnonhumanprimates |