Cargando…

Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice

Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice wer...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Ci, Guo, Nana, Bu, Yue, Peng, Yahui, Li, Xueting, Ma, Xiaohui, Yang, Mengyuan, Jia, Xiaonan, Zhang, Jin, Liu, Xiaowei, Yu, Kaijiang, Wang, Changsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064167/
https://www.ncbi.nlm.nih.gov/pubmed/33818419
http://dx.doi.org/10.18632/aging.202768
_version_ 1783682076971630592
author Han, Ci
Guo, Nana
Bu, Yue
Peng, Yahui
Li, Xueting
Ma, Xiaohui
Yang, Mengyuan
Jia, Xiaonan
Zhang, Jin
Liu, Xiaowei
Yu, Kaijiang
Wang, Changsong
author_facet Han, Ci
Guo, Nana
Bu, Yue
Peng, Yahui
Li, Xueting
Ma, Xiaohui
Yang, Mengyuan
Jia, Xiaonan
Zhang, Jin
Liu, Xiaowei
Yu, Kaijiang
Wang, Changsong
author_sort Han, Ci
collection PubMed
description Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection. Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them. Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis.
format Online
Article
Text
id pubmed-8064167
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-80641672021-04-26 Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice Han, Ci Guo, Nana Bu, Yue Peng, Yahui Li, Xueting Ma, Xiaohui Yang, Mengyuan Jia, Xiaonan Zhang, Jin Liu, Xiaowei Yu, Kaijiang Wang, Changsong Aging (Albany NY) Research Paper Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection. Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them. Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis. Impact Journals 2021-03-26 /pmc/articles/PMC8064167/ /pubmed/33818419 http://dx.doi.org/10.18632/aging.202768 Text en Copyright: © 2021 Han et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Han, Ci
Guo, Nana
Bu, Yue
Peng, Yahui
Li, Xueting
Ma, Xiaohui
Yang, Mengyuan
Jia, Xiaonan
Zhang, Jin
Liu, Xiaowei
Yu, Kaijiang
Wang, Changsong
Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title_full Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title_fullStr Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title_full_unstemmed Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title_short Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
title_sort intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064167/
https://www.ncbi.nlm.nih.gov/pubmed/33818419
http://dx.doi.org/10.18632/aging.202768
work_keys_str_mv AT hanci intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT guonana intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT buyue intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT pengyahui intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT lixueting intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT maxiaohui intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT yangmengyuan intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT jiaxiaonan intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT zhangjin intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT liuxiaowei intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT yukaijiang intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice
AT wangchangsong intestinalmicrobiotaandantibioticassociatedacutegastrointestinalinjuryinsepsismice