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Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice wer...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064167/ https://www.ncbi.nlm.nih.gov/pubmed/33818419 http://dx.doi.org/10.18632/aging.202768 |
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author | Han, Ci Guo, Nana Bu, Yue Peng, Yahui Li, Xueting Ma, Xiaohui Yang, Mengyuan Jia, Xiaonan Zhang, Jin Liu, Xiaowei Yu, Kaijiang Wang, Changsong |
author_facet | Han, Ci Guo, Nana Bu, Yue Peng, Yahui Li, Xueting Ma, Xiaohui Yang, Mengyuan Jia, Xiaonan Zhang, Jin Liu, Xiaowei Yu, Kaijiang Wang, Changsong |
author_sort | Han, Ci |
collection | PubMed |
description | Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection. Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them. Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis. |
format | Online Article Text |
id | pubmed-8064167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-80641672021-04-26 Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice Han, Ci Guo, Nana Bu, Yue Peng, Yahui Li, Xueting Ma, Xiaohui Yang, Mengyuan Jia, Xiaonan Zhang, Jin Liu, Xiaowei Yu, Kaijiang Wang, Changsong Aging (Albany NY) Research Paper Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection. Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them. Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis. Impact Journals 2021-03-26 /pmc/articles/PMC8064167/ /pubmed/33818419 http://dx.doi.org/10.18632/aging.202768 Text en Copyright: © 2021 Han et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Han, Ci Guo, Nana Bu, Yue Peng, Yahui Li, Xueting Ma, Xiaohui Yang, Mengyuan Jia, Xiaonan Zhang, Jin Liu, Xiaowei Yu, Kaijiang Wang, Changsong Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title | Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title_full | Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title_fullStr | Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title_full_unstemmed | Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title_short | Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
title_sort | intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064167/ https://www.ncbi.nlm.nih.gov/pubmed/33818419 http://dx.doi.org/10.18632/aging.202768 |
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