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Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo

The mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tu...

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Autores principales: Uruski, Paweł, Mikuła-Pietrasik, Justyna, Naumowicz, Eryk, Kaźmierczak, Kamila, Gaiday, Andrey N., Królak, Jan, Nowakowski, Błażej, Moszyński, Rafał, Tykarski, Andrzej, Książek, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064326/
https://www.ncbi.nlm.nih.gov/pubmed/33805246
http://dx.doi.org/10.3390/biomedicines9040330
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author Uruski, Paweł
Mikuła-Pietrasik, Justyna
Naumowicz, Eryk
Kaźmierczak, Kamila
Gaiday, Andrey N.
Królak, Jan
Nowakowski, Błażej
Moszyński, Rafał
Tykarski, Andrzej
Książek, Krzysztof
author_facet Uruski, Paweł
Mikuła-Pietrasik, Justyna
Naumowicz, Eryk
Kaźmierczak, Kamila
Gaiday, Andrey N.
Królak, Jan
Nowakowski, Błażej
Moszyński, Rafał
Tykarski, Andrzej
Książek, Krzysztof
author_sort Uruski, Paweł
collection PubMed
description The mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tumors obtained from ovarian cancer patients with respect to the expression of the senescence biomarkers SA-β-Gal and γ-H2A.X and the proliferative antigen Ki67. The results showed that the tumors displayed strong heterogeneity with respect to the expression of analyzed markers. The expression of SA-β-Gal and γ-H2A.X in the oldest patients (61–85 y.o.) was significantly higher than in the younger age groups. Conversely, the area of Ki67-positive cancer cells was greater in younger individuals. At the same time, there was a positive correlation between SA-β-Gal expression and calendar age in FIGO III–IV and malignant ascites-positive patients. The γ-H2A.X positively correlated with age in the whole group, FIGO III–IV, and ascites-positive patients. Ki67 levels correlated negatively with the age of patients among those same groups. Collectively, our study indicated that organismal aging may determine the development of the senescence phenotype in ovarian tumors, particularly in patients with advanced disease and those accumulating malignant ascites.
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spelling pubmed-80643262021-04-24 Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo Uruski, Paweł Mikuła-Pietrasik, Justyna Naumowicz, Eryk Kaźmierczak, Kamila Gaiday, Andrey N. Królak, Jan Nowakowski, Błażej Moszyński, Rafał Tykarski, Andrzej Książek, Krzysztof Biomedicines Article The mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tumors obtained from ovarian cancer patients with respect to the expression of the senescence biomarkers SA-β-Gal and γ-H2A.X and the proliferative antigen Ki67. The results showed that the tumors displayed strong heterogeneity with respect to the expression of analyzed markers. The expression of SA-β-Gal and γ-H2A.X in the oldest patients (61–85 y.o.) was significantly higher than in the younger age groups. Conversely, the area of Ki67-positive cancer cells was greater in younger individuals. At the same time, there was a positive correlation between SA-β-Gal expression and calendar age in FIGO III–IV and malignant ascites-positive patients. The γ-H2A.X positively correlated with age in the whole group, FIGO III–IV, and ascites-positive patients. Ki67 levels correlated negatively with the age of patients among those same groups. Collectively, our study indicated that organismal aging may determine the development of the senescence phenotype in ovarian tumors, particularly in patients with advanced disease and those accumulating malignant ascites. MDPI 2021-03-24 /pmc/articles/PMC8064326/ /pubmed/33805246 http://dx.doi.org/10.3390/biomedicines9040330 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Uruski, Paweł
Mikuła-Pietrasik, Justyna
Naumowicz, Eryk
Kaźmierczak, Kamila
Gaiday, Andrey N.
Królak, Jan
Nowakowski, Błażej
Moszyński, Rafał
Tykarski, Andrzej
Książek, Krzysztof
Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title_full Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title_fullStr Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title_full_unstemmed Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title_short Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
title_sort patient-specific variables determine the extent of cellular senescence biomarkers in ovarian tumors in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064326/
https://www.ncbi.nlm.nih.gov/pubmed/33805246
http://dx.doi.org/10.3390/biomedicines9040330
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