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Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064388/ https://www.ncbi.nlm.nih.gov/pubmed/33805173 http://dx.doi.org/10.3390/microorganisms9040674 |
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author | Leon, Fanny Pinchon, Elena Temurok, Nevzat Morvan, François Vasseur, Jean-Jacques Clot, Martine Foulongne, Vincent Cantaloube, Jean-François Vande Perre, Philippe Molès, Jean-Pierre Daynès, Aurélien Fournier-Wirth, Chantal |
author_facet | Leon, Fanny Pinchon, Elena Temurok, Nevzat Morvan, François Vasseur, Jean-Jacques Clot, Martine Foulongne, Vincent Cantaloube, Jean-François Vande Perre, Philippe Molès, Jean-Pierre Daynès, Aurélien Fournier-Wirth, Chantal |
author_sort | Leon, Fanny |
collection | PubMed |
description | Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections. |
format | Online Article Text |
id | pubmed-8064388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80643882021-04-24 Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection Leon, Fanny Pinchon, Elena Temurok, Nevzat Morvan, François Vasseur, Jean-Jacques Clot, Martine Foulongne, Vincent Cantaloube, Jean-François Vande Perre, Philippe Molès, Jean-Pierre Daynès, Aurélien Fournier-Wirth, Chantal Microorganisms Brief Report Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections. MDPI 2021-03-24 /pmc/articles/PMC8064388/ /pubmed/33805173 http://dx.doi.org/10.3390/microorganisms9040674 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Brief Report Leon, Fanny Pinchon, Elena Temurok, Nevzat Morvan, François Vasseur, Jean-Jacques Clot, Martine Foulongne, Vincent Cantaloube, Jean-François Vande Perre, Philippe Molès, Jean-Pierre Daynès, Aurélien Fournier-Wirth, Chantal Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title_full | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title_fullStr | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title_full_unstemmed | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title_short | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
title_sort | diagnostic performance of a magnetic field-enhanced agglutination readout in detecting either viral genomes or host antibodies in arbovirus infection |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064388/ https://www.ncbi.nlm.nih.gov/pubmed/33805173 http://dx.doi.org/10.3390/microorganisms9040674 |
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