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Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection

Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or...

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Autores principales: Leon, Fanny, Pinchon, Elena, Temurok, Nevzat, Morvan, François, Vasseur, Jean-Jacques, Clot, Martine, Foulongne, Vincent, Cantaloube, Jean-François, Vande Perre, Philippe, Molès, Jean-Pierre, Daynès, Aurélien, Fournier-Wirth, Chantal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064388/
https://www.ncbi.nlm.nih.gov/pubmed/33805173
http://dx.doi.org/10.3390/microorganisms9040674
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author Leon, Fanny
Pinchon, Elena
Temurok, Nevzat
Morvan, François
Vasseur, Jean-Jacques
Clot, Martine
Foulongne, Vincent
Cantaloube, Jean-François
Vande Perre, Philippe
Molès, Jean-Pierre
Daynès, Aurélien
Fournier-Wirth, Chantal
author_facet Leon, Fanny
Pinchon, Elena
Temurok, Nevzat
Morvan, François
Vasseur, Jean-Jacques
Clot, Martine
Foulongne, Vincent
Cantaloube, Jean-François
Vande Perre, Philippe
Molès, Jean-Pierre
Daynès, Aurélien
Fournier-Wirth, Chantal
author_sort Leon, Fanny
collection PubMed
description Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
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spelling pubmed-80643882021-04-24 Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection Leon, Fanny Pinchon, Elena Temurok, Nevzat Morvan, François Vasseur, Jean-Jacques Clot, Martine Foulongne, Vincent Cantaloube, Jean-François Vande Perre, Philippe Molès, Jean-Pierre Daynès, Aurélien Fournier-Wirth, Chantal Microorganisms Brief Report Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections. MDPI 2021-03-24 /pmc/articles/PMC8064388/ /pubmed/33805173 http://dx.doi.org/10.3390/microorganisms9040674 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Brief Report
Leon, Fanny
Pinchon, Elena
Temurok, Nevzat
Morvan, François
Vasseur, Jean-Jacques
Clot, Martine
Foulongne, Vincent
Cantaloube, Jean-François
Vande Perre, Philippe
Molès, Jean-Pierre
Daynès, Aurélien
Fournier-Wirth, Chantal
Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title_full Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title_fullStr Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title_full_unstemmed Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title_short Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
title_sort diagnostic performance of a magnetic field-enhanced agglutination readout in detecting either viral genomes or host antibodies in arbovirus infection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064388/
https://www.ncbi.nlm.nih.gov/pubmed/33805173
http://dx.doi.org/10.3390/microorganisms9040674
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