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Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit

SIMPLE SUMMARY: Lactate is a metabolite used in animal research as an indicator of muscle fatigue; therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate...

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Autores principales: Meléndez, Daniela M., Marti, Sonia, Faucitano, Luigi, Haley, Derek B., Schwinghamer, Timothy D., Schwartzkopf-Genswein, Karen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064460/
https://www.ncbi.nlm.nih.gov/pubmed/33806010
http://dx.doi.org/10.3390/ani11040926
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author Meléndez, Daniela M.
Marti, Sonia
Faucitano, Luigi
Haley, Derek B.
Schwinghamer, Timothy D.
Schwartzkopf-Genswein, Karen S.
author_facet Meléndez, Daniela M.
Marti, Sonia
Faucitano, Luigi
Haley, Derek B.
Schwinghamer, Timothy D.
Schwartzkopf-Genswein, Karen S.
author_sort Meléndez, Daniela M.
collection PubMed
description SIMPLE SUMMARY: Lactate is a metabolite used in animal research as an indicator of muscle fatigue; therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ hand-held analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected from 96 steers prior to loading and after 36 h of transportation, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ hand-held analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Correlations were calculated to assess the strength of the relationship between the L-lactate concentrations measured between methods. The strength of the correlation and the level of statistical significance varied over the observed time points, while the correlation for the pooled data was weak. Based on the low strength of the correlation, the Lactate Scout+ analyzer is not a suitable alternative to a colorimetric assay for measuring L-lactate in transported cattle. ABSTRACT: Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = −0.03 (p = 0.75; LO1) to r = 0.75 (p < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.
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spelling pubmed-80644602021-04-24 Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit Meléndez, Daniela M. Marti, Sonia Faucitano, Luigi Haley, Derek B. Schwinghamer, Timothy D. Schwartzkopf-Genswein, Karen S. Animals (Basel) Communication SIMPLE SUMMARY: Lactate is a metabolite used in animal research as an indicator of muscle fatigue; therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ hand-held analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected from 96 steers prior to loading and after 36 h of transportation, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ hand-held analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Correlations were calculated to assess the strength of the relationship between the L-lactate concentrations measured between methods. The strength of the correlation and the level of statistical significance varied over the observed time points, while the correlation for the pooled data was weak. Based on the low strength of the correlation, the Lactate Scout+ analyzer is not a suitable alternative to a colorimetric assay for measuring L-lactate in transported cattle. ABSTRACT: Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = −0.03 (p = 0.75; LO1) to r = 0.75 (p < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle. MDPI 2021-03-25 /pmc/articles/PMC8064460/ /pubmed/33806010 http://dx.doi.org/10.3390/ani11040926 Text en © Her Majesty the Queen in Right of Canada as represented by the Minister of Agriculture and Agri-Food, 2021 and the authors; https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Communication
Meléndez, Daniela M.
Marti, Sonia
Faucitano, Luigi
Haley, Derek B.
Schwinghamer, Timothy D.
Schwartzkopf-Genswein, Karen S.
Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title_full Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title_fullStr Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title_full_unstemmed Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title_short Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit
title_sort correlation between l-lactate concentrations in beef cattle, obtained using a hand-held lactate analyzer and a lactate assay colorimetric kit
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064460/
https://www.ncbi.nlm.nih.gov/pubmed/33806010
http://dx.doi.org/10.3390/ani11040926
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