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Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients

Metabolomics analysis of blood from patients (n = 42) undergoing surgery for suspected lung cancer was performed in this study. Venous and arterial blood was collected in both Streck and Heparin tubes. A total of 96 metabolites were detected, affected by sex (n = 56), collection tube (n = 33), and b...

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Autores principales: Goldberg, Erin, Ievari-Shariati, Shiva, Kidane, Biniam, Kim, Julian, Banerji, Shantanu, Qing, Gefei, Srinathan, Sadeesh, Murphy, Leigh, Aliani, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064553/
https://www.ncbi.nlm.nih.gov/pubmed/33891605
http://dx.doi.org/10.1371/journal.pone.0249648
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author Goldberg, Erin
Ievari-Shariati, Shiva
Kidane, Biniam
Kim, Julian
Banerji, Shantanu
Qing, Gefei
Srinathan, Sadeesh
Murphy, Leigh
Aliani, Michel
author_facet Goldberg, Erin
Ievari-Shariati, Shiva
Kidane, Biniam
Kim, Julian
Banerji, Shantanu
Qing, Gefei
Srinathan, Sadeesh
Murphy, Leigh
Aliani, Michel
author_sort Goldberg, Erin
collection PubMed
description Metabolomics analysis of blood from patients (n = 42) undergoing surgery for suspected lung cancer was performed in this study. Venous and arterial blood was collected in both Streck and Heparin tubes. A total of 96 metabolites were detected, affected by sex (n = 56), collection tube (n = 33), and blood location (n = 8). These metabolites belonged to a wide array of compound classes including lipids, acids, pharmaceutical agents, signalling molecules, vitamins, among others. Phospholipids and carboxylic acids accounted for 28% of all detected compounds. Out of the 33 compounds significantly affected by collection tube, 18 compounds were higher in the Streck tubes, including allantoin and ketoleucine, and 15 were higher in the Heparin tubes, including LysoPC(P-16:0), PS 40:6, and chenodeoxycholic acid glycine conjugate. Based on our results, it is recommended that replicate blood samples from each patient should be collected in different types of blood collection tubes for a broader range of the metabolome. Several metabolites were found at higher concentrations in cancer patients such as lactic acid in Squamous Cell Carcinoma, and lysoPCs in Adenocarcinoma and Acinar Cell Carcinoma, which may be used to detect early onset and/or to monitor the progress of the cancer patients.
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spelling pubmed-80645532021-05-04 Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients Goldberg, Erin Ievari-Shariati, Shiva Kidane, Biniam Kim, Julian Banerji, Shantanu Qing, Gefei Srinathan, Sadeesh Murphy, Leigh Aliani, Michel PLoS One Research Article Metabolomics analysis of blood from patients (n = 42) undergoing surgery for suspected lung cancer was performed in this study. Venous and arterial blood was collected in both Streck and Heparin tubes. A total of 96 metabolites were detected, affected by sex (n = 56), collection tube (n = 33), and blood location (n = 8). These metabolites belonged to a wide array of compound classes including lipids, acids, pharmaceutical agents, signalling molecules, vitamins, among others. Phospholipids and carboxylic acids accounted for 28% of all detected compounds. Out of the 33 compounds significantly affected by collection tube, 18 compounds were higher in the Streck tubes, including allantoin and ketoleucine, and 15 were higher in the Heparin tubes, including LysoPC(P-16:0), PS 40:6, and chenodeoxycholic acid glycine conjugate. Based on our results, it is recommended that replicate blood samples from each patient should be collected in different types of blood collection tubes for a broader range of the metabolome. Several metabolites were found at higher concentrations in cancer patients such as lactic acid in Squamous Cell Carcinoma, and lysoPCs in Adenocarcinoma and Acinar Cell Carcinoma, which may be used to detect early onset and/or to monitor the progress of the cancer patients. Public Library of Science 2021-04-23 /pmc/articles/PMC8064553/ /pubmed/33891605 http://dx.doi.org/10.1371/journal.pone.0249648 Text en © 2021 Goldberg et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Goldberg, Erin
Ievari-Shariati, Shiva
Kidane, Biniam
Kim, Julian
Banerji, Shantanu
Qing, Gefei
Srinathan, Sadeesh
Murphy, Leigh
Aliani, Michel
Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title_full Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title_fullStr Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title_full_unstemmed Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title_short Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
title_sort comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064553/
https://www.ncbi.nlm.nih.gov/pubmed/33891605
http://dx.doi.org/10.1371/journal.pone.0249648
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