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Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility

Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By c...

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Autores principales: Dolega, Monika E, Monnier, Sylvain, Brunel, Benjamin, Joanny, Jean-François, Recho, Pierre, Cappello, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064752/
https://www.ncbi.nlm.nih.gov/pubmed/33704063
http://dx.doi.org/10.7554/eLife.63258
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author Dolega, Monika E
Monnier, Sylvain
Brunel, Benjamin
Joanny, Jean-François
Recho, Pierre
Cappello, Giovanni
author_facet Dolega, Monika E
Monnier, Sylvain
Brunel, Benjamin
Joanny, Jean-François
Recho, Pierre
Cappello, Giovanni
author_sort Dolega, Monika E
collection PubMed
description Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the aggregates growth and internal cell motility, while selective compressions of same magnitude have almost no effect. Both compressions alter the volume of individual cells in the same way over a shor-timescale, but, by draining the water out of the extracellular matrix, the global one imposes a residual compressive mechanical stress on the cells over a long-timescale, while the selective one does not. We conclude that the extracellular matrix is as a sensor that mechanically regulates cell proliferation and migration in a 3D environment.
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spelling pubmed-80647522021-04-29 Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility Dolega, Monika E Monnier, Sylvain Brunel, Benjamin Joanny, Jean-François Recho, Pierre Cappello, Giovanni eLife Physics of Living Systems Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the aggregates growth and internal cell motility, while selective compressions of same magnitude have almost no effect. Both compressions alter the volume of individual cells in the same way over a shor-timescale, but, by draining the water out of the extracellular matrix, the global one imposes a residual compressive mechanical stress on the cells over a long-timescale, while the selective one does not. We conclude that the extracellular matrix is as a sensor that mechanically regulates cell proliferation and migration in a 3D environment. eLife Sciences Publications, Ltd 2021-03-11 /pmc/articles/PMC8064752/ /pubmed/33704063 http://dx.doi.org/10.7554/eLife.63258 Text en © 2021, Dolega et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Dolega, Monika E
Monnier, Sylvain
Brunel, Benjamin
Joanny, Jean-François
Recho, Pierre
Cappello, Giovanni
Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title_full Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title_fullStr Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title_full_unstemmed Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title_short Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
title_sort extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064752/
https://www.ncbi.nlm.nih.gov/pubmed/33704063
http://dx.doi.org/10.7554/eLife.63258
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