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Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility
Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064752/ https://www.ncbi.nlm.nih.gov/pubmed/33704063 http://dx.doi.org/10.7554/eLife.63258 |
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author | Dolega, Monika E Monnier, Sylvain Brunel, Benjamin Joanny, Jean-François Recho, Pierre Cappello, Giovanni |
author_facet | Dolega, Monika E Monnier, Sylvain Brunel, Benjamin Joanny, Jean-François Recho, Pierre Cappello, Giovanni |
author_sort | Dolega, Monika E |
collection | PubMed |
description | Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the aggregates growth and internal cell motility, while selective compressions of same magnitude have almost no effect. Both compressions alter the volume of individual cells in the same way over a shor-timescale, but, by draining the water out of the extracellular matrix, the global one imposes a residual compressive mechanical stress on the cells over a long-timescale, while the selective one does not. We conclude that the extracellular matrix is as a sensor that mechanically regulates cell proliferation and migration in a 3D environment. |
format | Online Article Text |
id | pubmed-8064752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80647522021-04-29 Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility Dolega, Monika E Monnier, Sylvain Brunel, Benjamin Joanny, Jean-François Recho, Pierre Cappello, Giovanni eLife Physics of Living Systems Imposed deformations play an important role in morphogenesis and tissue homeostasis, both in normal and pathological conditions. To perceive mechanical perturbations of different types and magnitudes, tissues need appropriate detectors, with a compliance that matches the perturbation amplitude. By comparing results of selective osmotic compressions of CT26 mouse cells within multicellular aggregates and global aggregate compressions, we show that global compressions have a strong impact on the aggregates growth and internal cell motility, while selective compressions of same magnitude have almost no effect. Both compressions alter the volume of individual cells in the same way over a shor-timescale, but, by draining the water out of the extracellular matrix, the global one imposes a residual compressive mechanical stress on the cells over a long-timescale, while the selective one does not. We conclude that the extracellular matrix is as a sensor that mechanically regulates cell proliferation and migration in a 3D environment. eLife Sciences Publications, Ltd 2021-03-11 /pmc/articles/PMC8064752/ /pubmed/33704063 http://dx.doi.org/10.7554/eLife.63258 Text en © 2021, Dolega et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Physics of Living Systems Dolega, Monika E Monnier, Sylvain Brunel, Benjamin Joanny, Jean-François Recho, Pierre Cappello, Giovanni Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title | Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title_full | Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title_fullStr | Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title_full_unstemmed | Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title_short | Extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
title_sort | extracellular matrix in multicellular aggregates acts as a pressure sensor controlling cell proliferation and motility |
topic | Physics of Living Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064752/ https://www.ncbi.nlm.nih.gov/pubmed/33704063 http://dx.doi.org/10.7554/eLife.63258 |
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