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Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments
Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronizat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064755/ https://www.ncbi.nlm.nih.gov/pubmed/33749592 http://dx.doi.org/10.7554/eLife.64348 |
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author | Arbel-Goren, Rinat Buonfiglio, Valentina Di Patti, Francesca Camargo, Sergio Zhitnitsky, Anna Valladares, Ana Flores, Enrique Herrero, Antonia Fanelli, Duccio Stavans, Joel |
author_facet | Arbel-Goren, Rinat Buonfiglio, Valentina Di Patti, Francesca Camargo, Sergio Zhitnitsky, Anna Valladares, Ana Flores, Enrique Herrero, Antonia Fanelli, Duccio Stavans, Joel |
author_sort | Arbel-Goren, Rinat |
collection | PubMed |
description | Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronization and spatial coherence along filaments, clock coupling due to cell-cell communication, and gating of the cell cycle. Furthermore, we observed low-amplitude circadian oscillatory transcription of kai genes encoding the post-transcriptional core oscillatory circuit and high-amplitude oscillations of rpaA coding for the master regulator transducing the core clock output. Transcriptional oscillations of rpaA suggest an additional level of regulation. A stochastic one-dimensional toy model of coupled clock cores and their phosphorylation states shows that demographic noise can seed stochastic oscillations outside the region where deterministic limit cycles with circadian periods occur. The model reproduces the observed spatio-temporal coherence along filaments and provides a robust description of coupled circadian clocks in a multicellular organism. |
format | Online Article Text |
id | pubmed-8064755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80647552021-04-29 Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments Arbel-Goren, Rinat Buonfiglio, Valentina Di Patti, Francesca Camargo, Sergio Zhitnitsky, Anna Valladares, Ana Flores, Enrique Herrero, Antonia Fanelli, Duccio Stavans, Joel eLife Physics of Living Systems Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronization and spatial coherence along filaments, clock coupling due to cell-cell communication, and gating of the cell cycle. Furthermore, we observed low-amplitude circadian oscillatory transcription of kai genes encoding the post-transcriptional core oscillatory circuit and high-amplitude oscillations of rpaA coding for the master regulator transducing the core clock output. Transcriptional oscillations of rpaA suggest an additional level of regulation. A stochastic one-dimensional toy model of coupled clock cores and their phosphorylation states shows that demographic noise can seed stochastic oscillations outside the region where deterministic limit cycles with circadian periods occur. The model reproduces the observed spatio-temporal coherence along filaments and provides a robust description of coupled circadian clocks in a multicellular organism. eLife Sciences Publications, Ltd 2021-03-22 /pmc/articles/PMC8064755/ /pubmed/33749592 http://dx.doi.org/10.7554/eLife.64348 Text en © 2021, Arbel-Goren et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Physics of Living Systems Arbel-Goren, Rinat Buonfiglio, Valentina Di Patti, Francesca Camargo, Sergio Zhitnitsky, Anna Valladares, Ana Flores, Enrique Herrero, Antonia Fanelli, Duccio Stavans, Joel Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title | Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title_full | Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title_fullStr | Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title_full_unstemmed | Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title_short | Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments |
title_sort | robust, coherent, and synchronized circadian clock-controlled oscillations along anabaena filaments |
topic | Physics of Living Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064755/ https://www.ncbi.nlm.nih.gov/pubmed/33749592 http://dx.doi.org/10.7554/eLife.64348 |
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