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The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury
PURPOSE: To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). METHODS: OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n =...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064804/ https://www.ncbi.nlm.nih.gov/pubmed/33953964 http://dx.doi.org/10.1155/2021/5565178 |
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author | Zhao, Xiao Han, Han Song, Yinting Du, Mei Liao, Mengyu Dong, Xue Wang, Xiaohong Kuhn, Ferenc Hoskin, Annette Xu, Heping Yan, Hua |
author_facet | Zhao, Xiao Han, Han Song, Yinting Du, Mei Liao, Mengyu Dong, Xue Wang, Xiaohong Kuhn, Ferenc Hoskin, Annette Xu, Heping Yan, Hua |
author_sort | Zhao, Xiao |
collection | PubMed |
description | PURPOSE: To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). METHODS: OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n = 5), OGI group (n = 40), and intravitreal Ranibizumab and Conbercept (n = 15 each). Ranibizumab or Conbercept was injected into the vitreous at 0.5 hours, 3 days, or 7 days. Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. RESULTS: Vitreous levels of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased in rabbit eyes after OGI. Compared to eyes in OGI group, anti-VEGF treatments significantly reduced these growth factors and cytokines. Among the 7 eyes examined from each group for intravitreal proliferative changes, they were found in 7 of 7 (100%) in OGI group and were decreased by Ranibizumab and Conbercept to 5 of 7 (71.4%) and 4 of 7 (57.1%), respectively. Both Ranibizumab and Conbercept inhibited epiretinal scar formation at the wound site, with Conbercept showing the greatest effect (maximal length of scar (L), L(OGI) = 503 ± 82.44 μm, L(Ranibizumab) = 355 ± 43.66 μm, and L(Conbercept) = 250.33 ± 36.02 μm). CONCLUSION: Anti-VEGF treatments after OGI significantly attenuated the upregulation of growth factors and cytokines in the vitreous and prevented intravitreal proliferation and epiretinal scar formation and thus may protect against the development of posttraumatic complications such as proliferative vitreoretinopathy (PVR). |
format | Online Article Text |
id | pubmed-8064804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-80648042021-05-04 The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury Zhao, Xiao Han, Han Song, Yinting Du, Mei Liao, Mengyu Dong, Xue Wang, Xiaohong Kuhn, Ferenc Hoskin, Annette Xu, Heping Yan, Hua J Ophthalmol Research Article PURPOSE: To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). METHODS: OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n = 5), OGI group (n = 40), and intravitreal Ranibizumab and Conbercept (n = 15 each). Ranibizumab or Conbercept was injected into the vitreous at 0.5 hours, 3 days, or 7 days. Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. RESULTS: Vitreous levels of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased in rabbit eyes after OGI. Compared to eyes in OGI group, anti-VEGF treatments significantly reduced these growth factors and cytokines. Among the 7 eyes examined from each group for intravitreal proliferative changes, they were found in 7 of 7 (100%) in OGI group and were decreased by Ranibizumab and Conbercept to 5 of 7 (71.4%) and 4 of 7 (57.1%), respectively. Both Ranibizumab and Conbercept inhibited epiretinal scar formation at the wound site, with Conbercept showing the greatest effect (maximal length of scar (L), L(OGI) = 503 ± 82.44 μm, L(Ranibizumab) = 355 ± 43.66 μm, and L(Conbercept) = 250.33 ± 36.02 μm). CONCLUSION: Anti-VEGF treatments after OGI significantly attenuated the upregulation of growth factors and cytokines in the vitreous and prevented intravitreal proliferation and epiretinal scar formation and thus may protect against the development of posttraumatic complications such as proliferative vitreoretinopathy (PVR). Hindawi 2021-04-15 /pmc/articles/PMC8064804/ /pubmed/33953964 http://dx.doi.org/10.1155/2021/5565178 Text en Copyright © 2021 Xiao Zhao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhao, Xiao Han, Han Song, Yinting Du, Mei Liao, Mengyu Dong, Xue Wang, Xiaohong Kuhn, Ferenc Hoskin, Annette Xu, Heping Yan, Hua The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title_full | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title_fullStr | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title_full_unstemmed | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title_short | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
title_sort | role of intravitreal anti-vegf agents in rabbit eye model of open-globe injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064804/ https://www.ncbi.nlm.nih.gov/pubmed/33953964 http://dx.doi.org/10.1155/2021/5565178 |
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