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Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering

Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study, the effect of cryopreservation on viability, stemness, and osteogenic differentiation capability of spontaneous CB-MSC spheroids were i...

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Autores principales: Dong, Hongwei, Li, Xianqi, Chen, Kai, Li, Ni, Kagami, Hideaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064946/
https://www.ncbi.nlm.nih.gov/pubmed/33798009
http://dx.doi.org/10.1089/ten.tec.2021.0001
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author Dong, Hongwei
Li, Xianqi
Chen, Kai
Li, Ni
Kagami, Hideaki
author_facet Dong, Hongwei
Li, Xianqi
Chen, Kai
Li, Ni
Kagami, Hideaki
author_sort Dong, Hongwei
collection PubMed
description Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study, the effect of cryopreservation on viability, stemness, and osteogenic differentiation capability of spontaneous CB-MSC spheroids were investigated. CB-MSCs were isolated from mouse femur and tibia. Spheroids were cryopreserved with various concentrations of dimethyl sulfoxide (DMSO). After thawing, the number of living and dead cells was measured. The expression levels of stem cell markers and osteogenic marker genes were analyzed. The cryopreserved and noncryopreserved spheroids were transplanted in mice with a beta-tricalcium phosphate as a scaffold to evaluate the in vivo bone-forming capability. The percentage of living cells was highest when 5% DMSO was used as a cryoprotectant, confirmed by the number of dead cells. The expression of stem cell marker genes and osteogenic differentiation capability were maintained after cryopreservation with 5% DMSO. The cryopreserved spontaneous CB-MSC spheroids showed remarkable new bone formation in vivo, identical to that of the noncryopreserved spheroids even without osteogenic induction. The cryopreserved spontaneous CB-MSC spheroids retained stemness and osteogenic differentiation capability and highlight the utility of spontaneous CB-MSC spheroids as ready-to-use tissue-engineered products for bone tissue engineering. IMPACT STATEMENT: The optimal dimethyl sulfoxide concentration for compact bone-derived mesenchymal stromal cells (CB-MSC) spheroid cryopreservation was determined. Cryopreservation did not affect the stemness and osteogenic capability of CB-MSC spheroids. Cryopreserved CB-MSC spheroids can be used for bone tissue engineering immediately after thawing without osteogenic induction.
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spelling pubmed-80649462021-04-26 Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering Dong, Hongwei Li, Xianqi Chen, Kai Li, Ni Kagami, Hideaki Tissue Eng Part C Methods Methods Articles Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study, the effect of cryopreservation on viability, stemness, and osteogenic differentiation capability of spontaneous CB-MSC spheroids were investigated. CB-MSCs were isolated from mouse femur and tibia. Spheroids were cryopreserved with various concentrations of dimethyl sulfoxide (DMSO). After thawing, the number of living and dead cells was measured. The expression levels of stem cell markers and osteogenic marker genes were analyzed. The cryopreserved and noncryopreserved spheroids were transplanted in mice with a beta-tricalcium phosphate as a scaffold to evaluate the in vivo bone-forming capability. The percentage of living cells was highest when 5% DMSO was used as a cryoprotectant, confirmed by the number of dead cells. The expression of stem cell marker genes and osteogenic differentiation capability were maintained after cryopreservation with 5% DMSO. The cryopreserved spontaneous CB-MSC spheroids showed remarkable new bone formation in vivo, identical to that of the noncryopreserved spheroids even without osteogenic induction. The cryopreserved spontaneous CB-MSC spheroids retained stemness and osteogenic differentiation capability and highlight the utility of spontaneous CB-MSC spheroids as ready-to-use tissue-engineered products for bone tissue engineering. IMPACT STATEMENT: The optimal dimethyl sulfoxide concentration for compact bone-derived mesenchymal stromal cells (CB-MSC) spheroid cryopreservation was determined. Cryopreservation did not affect the stemness and osteogenic capability of CB-MSC spheroids. Cryopreserved CB-MSC spheroids can be used for bone tissue engineering immediately after thawing without osteogenic induction. Mary Ann Liebert, Inc., publishers 2021-04-01 2021-04-19 /pmc/articles/PMC8064946/ /pubmed/33798009 http://dx.doi.org/10.1089/ten.tec.2021.0001 Text en © Hongwei Dong et al. 2021; Published by Mary Ann Liebert, Inc. https://creativecommons.org/licenses/by/4.0/This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Articles
Dong, Hongwei
Li, Xianqi
Chen, Kai
Li, Ni
Kagami, Hideaki
Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title_full Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title_fullStr Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title_full_unstemmed Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title_short Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
title_sort cryopreserved spontaneous spheroids from compact bone-derived mesenchymal stromal cells for bone tissue engineering
topic Methods Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064946/
https://www.ncbi.nlm.nih.gov/pubmed/33798009
http://dx.doi.org/10.1089/ten.tec.2021.0001
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