MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT

Previous research has found that miRNA-20b is highly expressed in gastric cancer (GC), however, its function and underlying mechanism are not clear. Wnt signaling pathway, implicated in tumorigeneisis, is activated in more than 30% of GC. We would like to characterize the biological behavior of miRN...

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Detalles Bibliográficos
Autores principales: Peng, Yin, Qin, Ying, Zhang, Xiaojing, Deng, Shiqi, Yuan, Yuan, Feng, Xianling, Chen, Wangchun, Hu, Fan, Gao, Yuli, He, Jieqiong, Cheng, Yulan, Wei, Yanjie, Fan, Xinmin, Ashktorab, Hassan, Smoot, Duane, Li, Song, Meltzer, Stephen J., Zhuang, Shutong, Tang, Na, Jin, Zhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065298/
https://www.ncbi.nlm.nih.gov/pubmed/33912703
http://dx.doi.org/10.1016/j.heliyon.2021.e06695
Descripción
Sumario:Previous research has found that miRNA-20b is highly expressed in gastric cancer (GC), however, its function and underlying mechanism are not clear. Wnt signaling pathway, implicated in tumorigeneisis, is activated in more than 30% of GC. We would like to characterize the biological behavior of miRNA-20b in terms of modulating Wnt/β-catenin signaling and EMT. We showed that miRNA-20b inhibitors suppressed Topflash/Fopflash dependent luciferase activity and the β-catenin nuclear translocation, resulting in inhibition of Wnt pathway activity and EMT. SUFU, negatively regulating Wnt and Hedgehog signaling pathway, was proved to be targeted by miRNA-20b. Moreover, additional knockdown of SUFU alleviated the inhibitory effect on Wnt pathway activity, EMT, cell proliferation/migration and colony formation caused by miRNA-20b inhibition. In summary, miRNA-20b is an oncogenic miRNA and promoted cell proliferation, migration and EMT in GC partially by activating Wnt pathway via targeting SUFU.

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