Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro tite...

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Autores principales: Lohse, Mirko, Thesen, Manuel W., Haase, Anja, Smolka, Martin, Iceta, Nerea Briz, Ayerdi Izquierdo, Ana, Ramos, Isbaal, Salado, Clarisa, Schleunitz, Arne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065385/
https://www.ncbi.nlm.nih.gov/pubmed/33916037
http://dx.doi.org/10.3390/nano11040902
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author Lohse, Mirko
Thesen, Manuel W.
Haase, Anja
Smolka, Martin
Iceta, Nerea Briz
Ayerdi Izquierdo, Ana
Ramos, Isbaal
Salado, Clarisa
Schleunitz, Arne
author_facet Lohse, Mirko
Thesen, Manuel W.
Haase, Anja
Smolka, Martin
Iceta, Nerea Briz
Ayerdi Izquierdo, Ana
Ramos, Isbaal
Salado, Clarisa
Schleunitz, Arne
author_sort Lohse, Mirko
collection PubMed
description The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.
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spelling pubmed-80653852021-04-25 Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out Lohse, Mirko Thesen, Manuel W. Haase, Anja Smolka, Martin Iceta, Nerea Briz Ayerdi Izquierdo, Ana Ramos, Isbaal Salado, Clarisa Schleunitz, Arne Nanomaterials (Basel) Article The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening. MDPI 2021-04-01 /pmc/articles/PMC8065385/ /pubmed/33916037 http://dx.doi.org/10.3390/nano11040902 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lohse, Mirko
Thesen, Manuel W.
Haase, Anja
Smolka, Martin
Iceta, Nerea Briz
Ayerdi Izquierdo, Ana
Ramos, Isbaal
Salado, Clarisa
Schleunitz, Arne
Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title_full Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title_fullStr Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title_full_unstemmed Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title_short Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out
title_sort novel concept of micro patterned micro titer plates fabricated via uv-nil for automated neuronal cell assay read-out
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065385/
https://www.ncbi.nlm.nih.gov/pubmed/33916037
http://dx.doi.org/10.3390/nano11040902
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