Cargando…
Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification
Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over t...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065410/ https://www.ncbi.nlm.nih.gov/pubmed/33801673 http://dx.doi.org/10.3390/metabo11040200 |
_version_ | 1783682335148867584 |
---|---|
author | Taylor, Michael J. Mattson, Sara Liyu, Andrey Stopka, Sylwia A. Ibrahim, Yehia M. Vertes, Akos Anderton, Christopher R. |
author_facet | Taylor, Michael J. Mattson, Sara Liyu, Andrey Stopka, Sylwia A. Ibrahim, Yehia M. Vertes, Akos Anderton, Christopher R. |
author_sort | Taylor, Michael J. |
collection | PubMed |
description | Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has been validated that this ionization technique is advantageous for probing the molecular content of individual cells in situ. Here, we report the integration of a microscope into the optical train of the LAESI source to allow for visually informed ambient in situ single cell analysis. Additionally, we have coupled this ‘LAESI microscope’ to a drift-tube ion mobility mass spectrometer to enable separation of isobaric species and allow for the determination of ion collision cross sections in conjunction with accurate mass measurements. This combined information helps provide higher confidence for structural assignment of molecules ablated from single cells. Here, we show that this system enables the analysis of the metabolite content of Allium cepa epidermal cells with high confidence structural identification together with their spatial locations within a tissue. |
format | Online Article Text |
id | pubmed-8065410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80654102021-04-25 Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification Taylor, Michael J. Mattson, Sara Liyu, Andrey Stopka, Sylwia A. Ibrahim, Yehia M. Vertes, Akos Anderton, Christopher R. Metabolites Article Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has been validated that this ionization technique is advantageous for probing the molecular content of individual cells in situ. Here, we report the integration of a microscope into the optical train of the LAESI source to allow for visually informed ambient in situ single cell analysis. Additionally, we have coupled this ‘LAESI microscope’ to a drift-tube ion mobility mass spectrometer to enable separation of isobaric species and allow for the determination of ion collision cross sections in conjunction with accurate mass measurements. This combined information helps provide higher confidence for structural assignment of molecules ablated from single cells. Here, we show that this system enables the analysis of the metabolite content of Allium cepa epidermal cells with high confidence structural identification together with their spatial locations within a tissue. MDPI 2021-03-27 /pmc/articles/PMC8065410/ /pubmed/33801673 http://dx.doi.org/10.3390/metabo11040200 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Taylor, Michael J. Mattson, Sara Liyu, Andrey Stopka, Sylwia A. Ibrahim, Yehia M. Vertes, Akos Anderton, Christopher R. Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title | Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title_full | Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title_fullStr | Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title_full_unstemmed | Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title_short | Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification |
title_sort | optical microscopy-guided laser ablation electrospray ionization ion mobility mass spectrometry: ambient single cell metabolomics with increased confidence in molecular identification |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065410/ https://www.ncbi.nlm.nih.gov/pubmed/33801673 http://dx.doi.org/10.3390/metabo11040200 |
work_keys_str_mv | AT taylormichaelj opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT mattsonsara opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT liyuandrey opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT stopkasylwiaa opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT ibrahimyehiam opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT vertesakos opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification AT andertonchristopherr opticalmicroscopyguidedlaserablationelectrosprayionizationionmobilitymassspectrometryambientsinglecellmetabolomicswithincreasedconfidenceinmolecularidentification |