Molecular Characteristics of Fat Body Protein 1 in the Oriental Fruit Fly, Bactrocera dorsalis

SIMPLE SUMMARY: Bactrocera dorsalis fat body protein 1 (Bdfbp1) cDNA was cloned. The deduced amino acid sequence contains three motifs: hemocyanin N, high molecular weight glutenin (gultenin hmw), and hemocyanin C from N to C termini. The glutenin hmw allows Bdfbp1 to fold into a compact form for storage. Bdfbp1 was highly expressed in the late third instar larvae and day 0 pupae. This suggests that Bdfbp1 is stored during larval stages as a storage protein for construction of adult tissues during pupal stages, and may be associated with adult eclosion. ABSTRACT: Bactrocera dorsails fat body protein 1 (Bdfbp1) cDNA was cloned (GenBank accession no. MT514270), and the complete 3,749-bp cDNA encoded a 1,152-amino acid protein. The phylogenetic relationship of dipteran fbp1s was analyzed. The sequence XP_028900815 from the insect genome project for Zeugodacus cucurbitae (LOC105219342) was proposed that two fbp1 genes were present in the sequence. The developmental transcriptional expression profiles were determined. In the larval stages, Bdfbp1 mRNA had significantly higher expression in the late third instar larvae compared with first, second, and early third instar larvae. In the pupal stages, the highest expression of Bdfbp1 mRNA was found in the newly pupated pupae and then decreased with age. In the fat body of female adults, Bdfbp1 was highly expressed in newly emerged samples and decreased rapidly over the following three days. In the fat body of male adults, Bdfbp1 was highly expressed in newly eclosed samples. RNAi treatment decreased the expression level of Bdfbp1 without statistical difference. However, RNAi treatment significantly decreased the rate of eclosion. These results suggest that Bdfbp1 may function as a storage protein and be associated with adult eclosion.