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Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome

Background: B lymphocytes (BL) seem to play an important role in transplantation, although the and role of different subpopulations in monitoring and outcome is not clear. Our aim was to monitoring immunological profiles based on BL subpopulations in kidney recipients (KR) with the risk of acute rej...

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Autores principales: Alfaro, Rafael, Legaz, Isabel, González-Martínez, Gema, Jimenez-Coll, Víctor, Martínez-Banaclocha, Helios, Galián, José Antonio, Botella, Carmen, de la Peña-Moral, Jesús, Moya-Quiles, María Rosa, Campillo, José Antonio, Minguela, Alfredo, Llorente, Santiago, Muro, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065535/
https://www.ncbi.nlm.nih.gov/pubmed/33916199
http://dx.doi.org/10.3390/diagnostics11040641
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author Alfaro, Rafael
Legaz, Isabel
González-Martínez, Gema
Jimenez-Coll, Víctor
Martínez-Banaclocha, Helios
Galián, José Antonio
Botella, Carmen
de la Peña-Moral, Jesús
Moya-Quiles, María Rosa
Campillo, José Antonio
Minguela, Alfredo
Llorente, Santiago
Muro, Manuel
author_facet Alfaro, Rafael
Legaz, Isabel
González-Martínez, Gema
Jimenez-Coll, Víctor
Martínez-Banaclocha, Helios
Galián, José Antonio
Botella, Carmen
de la Peña-Moral, Jesús
Moya-Quiles, María Rosa
Campillo, José Antonio
Minguela, Alfredo
Llorente, Santiago
Muro, Manuel
author_sort Alfaro, Rafael
collection PubMed
description Background: B lymphocytes (BL) seem to play an important role in transplantation, although the and role of different subpopulations in monitoring and outcome is not clear. Our aim was to monitoring immunological profiles based on BL subpopulations in kidney recipients (KR) with the risk of acute rejection (AR). Methods: Monitoring of BL subpopulations was performed by flow cytometry in PBLs before transplantation and three and six months after transplantation (PTX). We used two methodological approaches, a traditional analysis, and a novel cluster analysis, to determine the association between BL subpopulations, AR incidence, and graft function. Results: After three months of PTX, KRs with a B phenotype enriched in transitional BL and plasmablasts had better kidney function and lower AR incidence. KRs with decreased transitional BL and plasmablasts were associated with lower kidney function and higher AR PTX. KRs that had an increase in transitional BL PTX had a better clinical outcome. The increase in transitory BL during PTX was also associated with an increase in Tregs. Indeed, KRs receiving thymoglobulin as induction therapy showed a slight decrease in the relative frequency of naive BLs after three months of PTX. Conclusion: The monitoring of BL subpopulations may serve as a non-invasive tool to improve immunological follow-up of patients after kidney transplantation. However, further studies are needed to confirm the obtained results, define cut-off values, and standardize more optimal and even custom/customized protocols.
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spelling pubmed-80655352021-04-25 Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome Alfaro, Rafael Legaz, Isabel González-Martínez, Gema Jimenez-Coll, Víctor Martínez-Banaclocha, Helios Galián, José Antonio Botella, Carmen de la Peña-Moral, Jesús Moya-Quiles, María Rosa Campillo, José Antonio Minguela, Alfredo Llorente, Santiago Muro, Manuel Diagnostics (Basel) Article Background: B lymphocytes (BL) seem to play an important role in transplantation, although the and role of different subpopulations in monitoring and outcome is not clear. Our aim was to monitoring immunological profiles based on BL subpopulations in kidney recipients (KR) with the risk of acute rejection (AR). Methods: Monitoring of BL subpopulations was performed by flow cytometry in PBLs before transplantation and three and six months after transplantation (PTX). We used two methodological approaches, a traditional analysis, and a novel cluster analysis, to determine the association between BL subpopulations, AR incidence, and graft function. Results: After three months of PTX, KRs with a B phenotype enriched in transitional BL and plasmablasts had better kidney function and lower AR incidence. KRs with decreased transitional BL and plasmablasts were associated with lower kidney function and higher AR PTX. KRs that had an increase in transitional BL PTX had a better clinical outcome. The increase in transitory BL during PTX was also associated with an increase in Tregs. Indeed, KRs receiving thymoglobulin as induction therapy showed a slight decrease in the relative frequency of naive BLs after three months of PTX. Conclusion: The monitoring of BL subpopulations may serve as a non-invasive tool to improve immunological follow-up of patients after kidney transplantation. However, further studies are needed to confirm the obtained results, define cut-off values, and standardize more optimal and even custom/customized protocols. MDPI 2021-04-01 /pmc/articles/PMC8065535/ /pubmed/33916199 http://dx.doi.org/10.3390/diagnostics11040641 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alfaro, Rafael
Legaz, Isabel
González-Martínez, Gema
Jimenez-Coll, Víctor
Martínez-Banaclocha, Helios
Galián, José Antonio
Botella, Carmen
de la Peña-Moral, Jesús
Moya-Quiles, María Rosa
Campillo, José Antonio
Minguela, Alfredo
Llorente, Santiago
Muro, Manuel
Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title_full Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title_fullStr Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title_full_unstemmed Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title_short Monitoring of B Cell in Kidney Transplantation: Development of a Novel Clusters Analysis and Role of Transitional B Cells in Transplant Outcome
title_sort monitoring of b cell in kidney transplantation: development of a novel clusters analysis and role of transitional b cells in transplant outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065535/
https://www.ncbi.nlm.nih.gov/pubmed/33916199
http://dx.doi.org/10.3390/diagnostics11040641
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