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Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants

Alkaline phosphatases (ALPs: EC 3.1.3.1) are ubiquitous enzymes and play crucial roles in the fundamental phosphate uptake and secretory processes. Although insects are regarded as the most diverse group of organisms, the current understanding of ALP roles in insects is limited. As one type of destr...

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Autores principales: Han, Wen-Hao, Zou, Chi, Qian, Li-Xin, Wang, Chao, Wang, Xiao-Wei, Liu, Yin-Quan, Wang, Xin-Ru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065556/
https://www.ncbi.nlm.nih.gov/pubmed/33805320
http://dx.doi.org/10.3390/genes12040497
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author Han, Wen-Hao
Zou, Chi
Qian, Li-Xin
Wang, Chao
Wang, Xiao-Wei
Liu, Yin-Quan
Wang, Xin-Ru
author_facet Han, Wen-Hao
Zou, Chi
Qian, Li-Xin
Wang, Chao
Wang, Xiao-Wei
Liu, Yin-Quan
Wang, Xin-Ru
author_sort Han, Wen-Hao
collection PubMed
description Alkaline phosphatases (ALPs: EC 3.1.3.1) are ubiquitous enzymes and play crucial roles in the fundamental phosphate uptake and secretory processes. Although insects are regarded as the most diverse group of organisms, the current understanding of ALP roles in insects is limited. As one type of destructive agricultural pest, whitefly Bemisia tabaci, a phloem feeder and invasive species, can cause extensive crop damage through feeding and transmitting plant diseases. In this study, we retrieved five ALP genes in MEAM1 whitefly, nine ALP genes in MED whitefly via comparative genomics approaches. Compared with nine other insects, whiteflies’ ALP gene family members did not undergo significant expansion during insect evolution, and whiteflies’ ALP genes were dispersed. Moreover, whiteflies’ ALP gene family was conserved among insects and emerged before speciation via phylogenetic analysis. Whiteflies’ ALP gene expression profiles presented that most ALP genes have different expression patterns after feeding on cotton or tobacco plants. Female/male MED whiteflies possessed higher ALP activities on both cotton and tobacco plants irrespective of sex, relative to MEAM1 whiteflies. Meanwhile, adult MED whiteflies possessed higher ALP activity in both whole insect and salivary samples, relative to MEAM1 whiteflies. We also found that both MED and MEAM1 whiteflies could upregulate ALP activities after feeding on cotton compared with feeding on tobacco plants. These findings demonstrated the functions of whiteflies ALPs and will assist the further study of the genomic evolution of insect ALPs.
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spelling pubmed-80655562021-04-25 Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants Han, Wen-Hao Zou, Chi Qian, Li-Xin Wang, Chao Wang, Xiao-Wei Liu, Yin-Quan Wang, Xin-Ru Genes (Basel) Article Alkaline phosphatases (ALPs: EC 3.1.3.1) are ubiquitous enzymes and play crucial roles in the fundamental phosphate uptake and secretory processes. Although insects are regarded as the most diverse group of organisms, the current understanding of ALP roles in insects is limited. As one type of destructive agricultural pest, whitefly Bemisia tabaci, a phloem feeder and invasive species, can cause extensive crop damage through feeding and transmitting plant diseases. In this study, we retrieved five ALP genes in MEAM1 whitefly, nine ALP genes in MED whitefly via comparative genomics approaches. Compared with nine other insects, whiteflies’ ALP gene family members did not undergo significant expansion during insect evolution, and whiteflies’ ALP genes were dispersed. Moreover, whiteflies’ ALP gene family was conserved among insects and emerged before speciation via phylogenetic analysis. Whiteflies’ ALP gene expression profiles presented that most ALP genes have different expression patterns after feeding on cotton or tobacco plants. Female/male MED whiteflies possessed higher ALP activities on both cotton and tobacco plants irrespective of sex, relative to MEAM1 whiteflies. Meanwhile, adult MED whiteflies possessed higher ALP activity in both whole insect and salivary samples, relative to MEAM1 whiteflies. We also found that both MED and MEAM1 whiteflies could upregulate ALP activities after feeding on cotton compared with feeding on tobacco plants. These findings demonstrated the functions of whiteflies ALPs and will assist the further study of the genomic evolution of insect ALPs. MDPI 2021-03-29 /pmc/articles/PMC8065556/ /pubmed/33805320 http://dx.doi.org/10.3390/genes12040497 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Han, Wen-Hao
Zou, Chi
Qian, Li-Xin
Wang, Chao
Wang, Xiao-Wei
Liu, Yin-Quan
Wang, Xin-Ru
Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title_full Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title_fullStr Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title_full_unstemmed Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title_short Functional Analysis of Alkaline Phosphatase in Whitefly Bemisia tabaci (Middle East Asia Minor 1 and Mediterranean) on Different Host Plants
title_sort functional analysis of alkaline phosphatase in whitefly bemisia tabaci (middle east asia minor 1 and mediterranean) on different host plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065556/
https://www.ncbi.nlm.nih.gov/pubmed/33805320
http://dx.doi.org/10.3390/genes12040497
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