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Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells
Tetraponera rufonigra (Arboreal Bicoloured Ant) venom induces pain, inflammation, and anaphylaxis in people and has an increased incident in Southeast Asia regions. The bioactive components and mechanism of action of the ant venom are still limited. The aim of this research was to identify the prote...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065575/ https://www.ncbi.nlm.nih.gov/pubmed/33916734 http://dx.doi.org/10.3390/toxins13040257 |
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author | Naephrai, Suwatjanee Khacha-ananda, Supakit Pitchakarn, Pornsiri Jaikang, Churdsak |
author_facet | Naephrai, Suwatjanee Khacha-ananda, Supakit Pitchakarn, Pornsiri Jaikang, Churdsak |
author_sort | Naephrai, Suwatjanee |
collection | PubMed |
description | Tetraponera rufonigra (Arboreal Bicoloured Ant) venom induces pain, inflammation, and anaphylaxis in people and has an increased incident in Southeast Asia regions. The bioactive components and mechanism of action of the ant venom are still limited. The aim of this research was to identify the protein composition and inflammatory process of the ant venom by using RAW 264.7 macrophage cells. The major venom proteins are composed of 5’ nucleotidase, prolyl endopeptidase-like, aminopeptidase N, trypsin-3, venom protein, and phospholipase A(2) (PLA(2)). The venom showed PLA(2) activity and represented 0.46 μg of PLA(2) bee venom equivalent/μg crude venom protein. The venom induced cytotoxic in a dose- and time-dependent manner with IC(20) approximately at 4.01 µg/mL. The increased levels of COX-2 and PGE(2) were observed after 1 h of treatment correlating with an upregulation of COX-2 expression. Moreover, the level of mPGES-1 expression was obviously increased after 12 h of venom induction. Hence, our results suggested that the induction of COX-2/mPGEs-1 pathway could be a direct pathway for the ant venom-induced inflammation. |
format | Online Article Text |
id | pubmed-8065575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80655752021-04-25 Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells Naephrai, Suwatjanee Khacha-ananda, Supakit Pitchakarn, Pornsiri Jaikang, Churdsak Toxins (Basel) Article Tetraponera rufonigra (Arboreal Bicoloured Ant) venom induces pain, inflammation, and anaphylaxis in people and has an increased incident in Southeast Asia regions. The bioactive components and mechanism of action of the ant venom are still limited. The aim of this research was to identify the protein composition and inflammatory process of the ant venom by using RAW 264.7 macrophage cells. The major venom proteins are composed of 5’ nucleotidase, prolyl endopeptidase-like, aminopeptidase N, trypsin-3, venom protein, and phospholipase A(2) (PLA(2)). The venom showed PLA(2) activity and represented 0.46 μg of PLA(2) bee venom equivalent/μg crude venom protein. The venom induced cytotoxic in a dose- and time-dependent manner with IC(20) approximately at 4.01 µg/mL. The increased levels of COX-2 and PGE(2) were observed after 1 h of treatment correlating with an upregulation of COX-2 expression. Moreover, the level of mPGES-1 expression was obviously increased after 12 h of venom induction. Hence, our results suggested that the induction of COX-2/mPGEs-1 pathway could be a direct pathway for the ant venom-induced inflammation. MDPI 2021-04-03 /pmc/articles/PMC8065575/ /pubmed/33916734 http://dx.doi.org/10.3390/toxins13040257 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Naephrai, Suwatjanee Khacha-ananda, Supakit Pitchakarn, Pornsiri Jaikang, Churdsak Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title | Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title_full | Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title_fullStr | Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title_full_unstemmed | Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title_short | Composition and Acute Inflammatory Response from Tetraponera rufonigra Venom on RAW 264.7 Macrophage Cells |
title_sort | composition and acute inflammatory response from tetraponera rufonigra venom on raw 264.7 macrophage cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065575/ https://www.ncbi.nlm.nih.gov/pubmed/33916734 http://dx.doi.org/10.3390/toxins13040257 |
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