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bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts

Tissue-engineered vascular grafts are widely tested as a promising substitute for both arterial bypass and replacement surgery. We previously demonstrated that incorporation of VEGF into electrospun tubular scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) enhances for...

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Autores principales: Antonova, Larisa, Kutikhin, Anton, Sevostianova, Viktoriia, Velikanova, Elena, Matveeva, Vera, Glushkova, Tatiana, Mironov, Andrey, Krivkina, Evgeniya, Shabaev, Amin, Senokosova, Evgeniya, Barbarash, Leonid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065794/
https://www.ncbi.nlm.nih.gov/pubmed/33800631
http://dx.doi.org/10.3390/ph14040302
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author Antonova, Larisa
Kutikhin, Anton
Sevostianova, Viktoriia
Velikanova, Elena
Matveeva, Vera
Glushkova, Tatiana
Mironov, Andrey
Krivkina, Evgeniya
Shabaev, Amin
Senokosova, Evgeniya
Barbarash, Leonid
author_facet Antonova, Larisa
Kutikhin, Anton
Sevostianova, Viktoriia
Velikanova, Elena
Matveeva, Vera
Glushkova, Tatiana
Mironov, Andrey
Krivkina, Evgeniya
Shabaev, Amin
Senokosova, Evgeniya
Barbarash, Leonid
author_sort Antonova, Larisa
collection PubMed
description Tissue-engineered vascular grafts are widely tested as a promising substitute for both arterial bypass and replacement surgery. We previously demonstrated that incorporation of VEGF into electrospun tubular scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) enhances formation of an endothelial cell monolayer. However, an overdose of VEGF can induce tumor-like vasculature; thereby, other bioactive factors are needed to support VEGF-driven endothelialization and successful recruitment of smooth muscle cells. Utilizing emulsion electrospinning, we fabricated one-layer vascular grafts with either VEGF, bFGF, or SDF-1α, and two-layer vascular grafts with VEGF incorporated into the inner layer and bFGF and SDF-1α incorporated into the outer layer with the following structural evaluation, tensile testing, and in vivo testing using a rat abdominal aorta replacement model. The latter graft prototype showed higher primary patency rate. We found that the two-layer structure improved surface topography and mechanical properties of the grafts. Further, the combination of bFGF, SDF-1α, and VEGF improved endothelialization compared with VEGF alone, while bFGF induced a rapid formation of a smooth muscle cell layer. Taken together, these findings show that the two-layer structure and incorporation of bFGF and SDF-1α into the vascular grafts in combination with VEGF provide a higher primary patency and therefore improved in vivo performance.
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spelling pubmed-80657942021-04-25 bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts Antonova, Larisa Kutikhin, Anton Sevostianova, Viktoriia Velikanova, Elena Matveeva, Vera Glushkova, Tatiana Mironov, Andrey Krivkina, Evgeniya Shabaev, Amin Senokosova, Evgeniya Barbarash, Leonid Pharmaceuticals (Basel) Article Tissue-engineered vascular grafts are widely tested as a promising substitute for both arterial bypass and replacement surgery. We previously demonstrated that incorporation of VEGF into electrospun tubular scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) enhances formation of an endothelial cell monolayer. However, an overdose of VEGF can induce tumor-like vasculature; thereby, other bioactive factors are needed to support VEGF-driven endothelialization and successful recruitment of smooth muscle cells. Utilizing emulsion electrospinning, we fabricated one-layer vascular grafts with either VEGF, bFGF, or SDF-1α, and two-layer vascular grafts with VEGF incorporated into the inner layer and bFGF and SDF-1α incorporated into the outer layer with the following structural evaluation, tensile testing, and in vivo testing using a rat abdominal aorta replacement model. The latter graft prototype showed higher primary patency rate. We found that the two-layer structure improved surface topography and mechanical properties of the grafts. Further, the combination of bFGF, SDF-1α, and VEGF improved endothelialization compared with VEGF alone, while bFGF induced a rapid formation of a smooth muscle cell layer. Taken together, these findings show that the two-layer structure and incorporation of bFGF and SDF-1α into the vascular grafts in combination with VEGF provide a higher primary patency and therefore improved in vivo performance. MDPI 2021-03-28 /pmc/articles/PMC8065794/ /pubmed/33800631 http://dx.doi.org/10.3390/ph14040302 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Antonova, Larisa
Kutikhin, Anton
Sevostianova, Viktoriia
Velikanova, Elena
Matveeva, Vera
Glushkova, Tatiana
Mironov, Andrey
Krivkina, Evgeniya
Shabaev, Amin
Senokosova, Evgeniya
Barbarash, Leonid
bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title_full bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title_fullStr bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title_full_unstemmed bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title_short bFGF and SDF-1α Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts
title_sort bfgf and sdf-1α improve in vivo performance of vegf-incorporating small-diameter vascular grafts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065794/
https://www.ncbi.nlm.nih.gov/pubmed/33800631
http://dx.doi.org/10.3390/ph14040302
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