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Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their pr...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065836/ https://www.ncbi.nlm.nih.gov/pubmed/33918274 http://dx.doi.org/10.3390/cells10040791 |
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author | Ruf, Wolfgang P. Freischmidt, Axel Grozdanov, Veselin Roth, Valerie Brockmann, Sarah J. Mollenhauer, Brit Martin, Dorothea Haslinger, Bernhard Fundel-Clemens, Katrin Otto, Markus von Arnim, Christine Holzmann, Karlheinz Ludolph, Albert C. Weishaupt, Jochen H. Danzer, Karin M. |
author_facet | Ruf, Wolfgang P. Freischmidt, Axel Grozdanov, Veselin Roth, Valerie Brockmann, Sarah J. Mollenhauer, Brit Martin, Dorothea Haslinger, Bernhard Fundel-Clemens, Katrin Otto, Markus von Arnim, Christine Holzmann, Karlheinz Ludolph, Albert C. Weishaupt, Jochen H. Danzer, Karin M. |
author_sort | Ruf, Wolfgang P. |
collection | PubMed |
description | Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their protein binding partners. We found significant alterations of the miRNA abundance pattern in serum and in isolated serum-derived extracellular vesicles of Parkinson’s disease (PD) patients. The differential expression of miRNA in PD patients was more robust in serum than in isolated extracellular vesicles and could separate PD patients from healthy controls in an unsupervised approach to a high degree. We identified a novel protein interaction partner for the strongly dysregulated hsa-mir-4745-5p. Our study provides further evidence for the involvement of miRNAs and HNF4a in PD. The demonstration that miRNA-protein binding might mediate the pathologic effects of HNF4a both by direct binding to it and by binding to proteins regulated by it suggests a complex role for miRNAs in pathology beyond the dysregulation of transcription. |
format | Online Article Text |
id | pubmed-8065836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80658362021-04-25 Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum Ruf, Wolfgang P. Freischmidt, Axel Grozdanov, Veselin Roth, Valerie Brockmann, Sarah J. Mollenhauer, Brit Martin, Dorothea Haslinger, Bernhard Fundel-Clemens, Katrin Otto, Markus von Arnim, Christine Holzmann, Karlheinz Ludolph, Albert C. Weishaupt, Jochen H. Danzer, Karin M. Cells Article Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their protein binding partners. We found significant alterations of the miRNA abundance pattern in serum and in isolated serum-derived extracellular vesicles of Parkinson’s disease (PD) patients. The differential expression of miRNA in PD patients was more robust in serum than in isolated extracellular vesicles and could separate PD patients from healthy controls in an unsupervised approach to a high degree. We identified a novel protein interaction partner for the strongly dysregulated hsa-mir-4745-5p. Our study provides further evidence for the involvement of miRNAs and HNF4a in PD. The demonstration that miRNA-protein binding might mediate the pathologic effects of HNF4a both by direct binding to it and by binding to proteins regulated by it suggests a complex role for miRNAs in pathology beyond the dysregulation of transcription. MDPI 2021-04-02 /pmc/articles/PMC8065836/ /pubmed/33918274 http://dx.doi.org/10.3390/cells10040791 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ruf, Wolfgang P. Freischmidt, Axel Grozdanov, Veselin Roth, Valerie Brockmann, Sarah J. Mollenhauer, Brit Martin, Dorothea Haslinger, Bernhard Fundel-Clemens, Katrin Otto, Markus von Arnim, Christine Holzmann, Karlheinz Ludolph, Albert C. Weishaupt, Jochen H. Danzer, Karin M. Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title | Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title_full | Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title_fullStr | Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title_full_unstemmed | Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title_short | Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum |
title_sort | protein binding partners of dysregulated mirnas in parkinson’s disease serum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065836/ https://www.ncbi.nlm.nih.gov/pubmed/33918274 http://dx.doi.org/10.3390/cells10040791 |
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