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Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum

Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their pr...

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Autores principales: Ruf, Wolfgang P., Freischmidt, Axel, Grozdanov, Veselin, Roth, Valerie, Brockmann, Sarah J., Mollenhauer, Brit, Martin, Dorothea, Haslinger, Bernhard, Fundel-Clemens, Katrin, Otto, Markus, von Arnim, Christine, Holzmann, Karlheinz, Ludolph, Albert C., Weishaupt, Jochen H., Danzer, Karin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065836/
https://www.ncbi.nlm.nih.gov/pubmed/33918274
http://dx.doi.org/10.3390/cells10040791
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author Ruf, Wolfgang P.
Freischmidt, Axel
Grozdanov, Veselin
Roth, Valerie
Brockmann, Sarah J.
Mollenhauer, Brit
Martin, Dorothea
Haslinger, Bernhard
Fundel-Clemens, Katrin
Otto, Markus
von Arnim, Christine
Holzmann, Karlheinz
Ludolph, Albert C.
Weishaupt, Jochen H.
Danzer, Karin M.
author_facet Ruf, Wolfgang P.
Freischmidt, Axel
Grozdanov, Veselin
Roth, Valerie
Brockmann, Sarah J.
Mollenhauer, Brit
Martin, Dorothea
Haslinger, Bernhard
Fundel-Clemens, Katrin
Otto, Markus
von Arnim, Christine
Holzmann, Karlheinz
Ludolph, Albert C.
Weishaupt, Jochen H.
Danzer, Karin M.
author_sort Ruf, Wolfgang P.
collection PubMed
description Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their protein binding partners. We found significant alterations of the miRNA abundance pattern in serum and in isolated serum-derived extracellular vesicles of Parkinson’s disease (PD) patients. The differential expression of miRNA in PD patients was more robust in serum than in isolated extracellular vesicles and could separate PD patients from healthy controls in an unsupervised approach to a high degree. We identified a novel protein interaction partner for the strongly dysregulated hsa-mir-4745-5p. Our study provides further evidence for the involvement of miRNAs and HNF4a in PD. The demonstration that miRNA-protein binding might mediate the pathologic effects of HNF4a both by direct binding to it and by binding to proteins regulated by it suggests a complex role for miRNAs in pathology beyond the dysregulation of transcription.
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spelling pubmed-80658362021-04-25 Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum Ruf, Wolfgang P. Freischmidt, Axel Grozdanov, Veselin Roth, Valerie Brockmann, Sarah J. Mollenhauer, Brit Martin, Dorothea Haslinger, Bernhard Fundel-Clemens, Katrin Otto, Markus von Arnim, Christine Holzmann, Karlheinz Ludolph, Albert C. Weishaupt, Jochen H. Danzer, Karin M. Cells Article Accumulating evidence suggests that microRNAs (miRNAs) are a contributing factor to neurodegenerative diseases. Although altered miRNA profiles in serum or plasma have been reported for several neurodegenerative diseases, little is known about the interaction between dysregulated miRNAs and their protein binding partners. We found significant alterations of the miRNA abundance pattern in serum and in isolated serum-derived extracellular vesicles of Parkinson’s disease (PD) patients. The differential expression of miRNA in PD patients was more robust in serum than in isolated extracellular vesicles and could separate PD patients from healthy controls in an unsupervised approach to a high degree. We identified a novel protein interaction partner for the strongly dysregulated hsa-mir-4745-5p. Our study provides further evidence for the involvement of miRNAs and HNF4a in PD. The demonstration that miRNA-protein binding might mediate the pathologic effects of HNF4a both by direct binding to it and by binding to proteins regulated by it suggests a complex role for miRNAs in pathology beyond the dysregulation of transcription. MDPI 2021-04-02 /pmc/articles/PMC8065836/ /pubmed/33918274 http://dx.doi.org/10.3390/cells10040791 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruf, Wolfgang P.
Freischmidt, Axel
Grozdanov, Veselin
Roth, Valerie
Brockmann, Sarah J.
Mollenhauer, Brit
Martin, Dorothea
Haslinger, Bernhard
Fundel-Clemens, Katrin
Otto, Markus
von Arnim, Christine
Holzmann, Karlheinz
Ludolph, Albert C.
Weishaupt, Jochen H.
Danzer, Karin M.
Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title_full Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title_fullStr Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title_full_unstemmed Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title_short Protein Binding Partners of Dysregulated miRNAs in Parkinson’s Disease Serum
title_sort protein binding partners of dysregulated mirnas in parkinson’s disease serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065836/
https://www.ncbi.nlm.nih.gov/pubmed/33918274
http://dx.doi.org/10.3390/cells10040791
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