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Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model

Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for mult...

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Autores principales: Rakov, Chani, Ben Porat, Shira, Alkalay-Oren, Sivan, Yerushalmy, Ortal, Abdalrhman, Mohanad, Gronovich, Niv, Huang, Lina, Pride, David, Coppenhagen-Glazer, Shunit, Nir-Paz, Ran, Hazan, Ronen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065852/
https://www.ncbi.nlm.nih.gov/pubmed/33918377
http://dx.doi.org/10.3390/antibiotics10040375
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author Rakov, Chani
Ben Porat, Shira
Alkalay-Oren, Sivan
Yerushalmy, Ortal
Abdalrhman, Mohanad
Gronovich, Niv
Huang, Lina
Pride, David
Coppenhagen-Glazer, Shunit
Nir-Paz, Ran
Hazan, Ronen
author_facet Rakov, Chani
Ben Porat, Shira
Alkalay-Oren, Sivan
Yerushalmy, Ortal
Abdalrhman, Mohanad
Gronovich, Niv
Huang, Lina
Pride, David
Coppenhagen-Glazer, Shunit
Nir-Paz, Ran
Hazan, Ronen
author_sort Rakov, Chani
collection PubMed
description Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular.
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spelling pubmed-80658522021-04-25 Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model Rakov, Chani Ben Porat, Shira Alkalay-Oren, Sivan Yerushalmy, Ortal Abdalrhman, Mohanad Gronovich, Niv Huang, Lina Pride, David Coppenhagen-Glazer, Shunit Nir-Paz, Ran Hazan, Ronen Antibiotics (Basel) Article Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular. MDPI 2021-04-02 /pmc/articles/PMC8065852/ /pubmed/33918377 http://dx.doi.org/10.3390/antibiotics10040375 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rakov, Chani
Ben Porat, Shira
Alkalay-Oren, Sivan
Yerushalmy, Ortal
Abdalrhman, Mohanad
Gronovich, Niv
Huang, Lina
Pride, David
Coppenhagen-Glazer, Shunit
Nir-Paz, Ran
Hazan, Ronen
Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title_full Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title_fullStr Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title_full_unstemmed Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title_short Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model
title_sort targeting biofilm of mdr providencia stuartii by phages using a catheter model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065852/
https://www.ncbi.nlm.nih.gov/pubmed/33918377
http://dx.doi.org/10.3390/antibiotics10040375
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