Cargando…

Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber

A one-pot and one-step enzymatic synthesis of submicron-order spherical microparticles composed of dehydrogenative polymers (DHPs) of coniferyl alcohol as a typical lignin precursor and TEMPO-oxidized cellulose nanofibers (TOCNFs) was investigated. Horseradish peroxidase enzymatically catalyzed the...

Descripción completa

Detalles Bibliográficos
Autores principales: Fukuda, Naoya, Hatakeyama, Mayumi, Kitaoka, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065862/
https://www.ncbi.nlm.nih.gov/pubmed/33916825
http://dx.doi.org/10.3390/nano11040917
_version_ 1783682440033730560
author Fukuda, Naoya
Hatakeyama, Mayumi
Kitaoka, Takuya
author_facet Fukuda, Naoya
Hatakeyama, Mayumi
Kitaoka, Takuya
author_sort Fukuda, Naoya
collection PubMed
description A one-pot and one-step enzymatic synthesis of submicron-order spherical microparticles composed of dehydrogenative polymers (DHPs) of coniferyl alcohol as a typical lignin precursor and TEMPO-oxidized cellulose nanofibers (TOCNFs) was investigated. Horseradish peroxidase enzymatically catalyzed the radical coupling of coniferyl alcohol in an aqueous suspension of TOCNFs, resulting in the formation of spherical microparticles with a diameter and sphericity index of approximately 0.8 μm and 0.95, respectively. The ζ-potential of TOCNF-functionalized DHP microspheres was about −40 mV, indicating that the colloidal systems had good stability. Nanofibrous components were clearly observed on the microparticle surface by scanning electron microscopy, while some TOCNFs were confirmed to be inside the microparticles by confocal laser scanning microscopy with Calcofluor white staining. As both cellulose and lignin are natural polymers known to biodegrade, even in the sea, these woody TOCNF−DHP microparticle nanocomposites were expected to be promising alternatives to fossil resource-derived microbeads in cosmetic applications.
format Online
Article
Text
id pubmed-8065862
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80658622021-04-25 Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber Fukuda, Naoya Hatakeyama, Mayumi Kitaoka, Takuya Nanomaterials (Basel) Article A one-pot and one-step enzymatic synthesis of submicron-order spherical microparticles composed of dehydrogenative polymers (DHPs) of coniferyl alcohol as a typical lignin precursor and TEMPO-oxidized cellulose nanofibers (TOCNFs) was investigated. Horseradish peroxidase enzymatically catalyzed the radical coupling of coniferyl alcohol in an aqueous suspension of TOCNFs, resulting in the formation of spherical microparticles with a diameter and sphericity index of approximately 0.8 μm and 0.95, respectively. The ζ-potential of TOCNF-functionalized DHP microspheres was about −40 mV, indicating that the colloidal systems had good stability. Nanofibrous components were clearly observed on the microparticle surface by scanning electron microscopy, while some TOCNFs were confirmed to be inside the microparticles by confocal laser scanning microscopy with Calcofluor white staining. As both cellulose and lignin are natural polymers known to biodegrade, even in the sea, these woody TOCNF−DHP microparticle nanocomposites were expected to be promising alternatives to fossil resource-derived microbeads in cosmetic applications. MDPI 2021-04-03 /pmc/articles/PMC8065862/ /pubmed/33916825 http://dx.doi.org/10.3390/nano11040917 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fukuda, Naoya
Hatakeyama, Mayumi
Kitaoka, Takuya
Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title_full Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title_fullStr Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title_full_unstemmed Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title_short Enzymatic Preparation and Characterization of Spherical Microparticles Composed of Artificial Lignin and TEMPO-Oxidized Cellulose Nanofiber
title_sort enzymatic preparation and characterization of spherical microparticles composed of artificial lignin and tempo-oxidized cellulose nanofiber
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065862/
https://www.ncbi.nlm.nih.gov/pubmed/33916825
http://dx.doi.org/10.3390/nano11040917
work_keys_str_mv AT fukudanaoya enzymaticpreparationandcharacterizationofsphericalmicroparticlescomposedofartificialligninandtempooxidizedcellulosenanofiber
AT hatakeyamamayumi enzymaticpreparationandcharacterizationofsphericalmicroparticlescomposedofartificialligninandtempooxidizedcellulosenanofiber
AT kitaokatakuya enzymaticpreparationandcharacterizationofsphericalmicroparticlescomposedofartificialligninandtempooxidizedcellulosenanofiber