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Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application

The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased imm...

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Autores principales: De Benedetti, Stefano, Di Pisa, Flavio, Fassi, Enrico Mario Alessandro, Cretich, Marina, Musicò, Angelo, Frigerio, Roberto, Mussida, Alessandro, Bombaci, Mauro, Grifantini, Renata, Colombo, Giorgio, Bolognesi, Martino, Grande, Romualdo, Zanchetta, Nadia, Gismondo, Maria Rita, Mileto, Davide, Mancon, Alessandro, Gourlay, Louise Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066017/
https://www.ncbi.nlm.nih.gov/pubmed/33915716
http://dx.doi.org/10.3390/vaccines9040322
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author De Benedetti, Stefano
Di Pisa, Flavio
Fassi, Enrico Mario Alessandro
Cretich, Marina
Musicò, Angelo
Frigerio, Roberto
Mussida, Alessandro
Bombaci, Mauro
Grifantini, Renata
Colombo, Giorgio
Bolognesi, Martino
Grande, Romualdo
Zanchetta, Nadia
Gismondo, Maria Rita
Mileto, Davide
Mancon, Alessandro
Gourlay, Louise Jane
author_facet De Benedetti, Stefano
Di Pisa, Flavio
Fassi, Enrico Mario Alessandro
Cretich, Marina
Musicò, Angelo
Frigerio, Roberto
Mussida, Alessandro
Bombaci, Mauro
Grifantini, Renata
Colombo, Giorgio
Bolognesi, Martino
Grande, Romualdo
Zanchetta, Nadia
Gismondo, Maria Rita
Mileto, Davide
Mancon, Alessandro
Gourlay, Louise Jane
author_sort De Benedetti, Stefano
collection PubMed
description The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
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spelling pubmed-80660172021-04-25 Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application De Benedetti, Stefano Di Pisa, Flavio Fassi, Enrico Mario Alessandro Cretich, Marina Musicò, Angelo Frigerio, Roberto Mussida, Alessandro Bombaci, Mauro Grifantini, Renata Colombo, Giorgio Bolognesi, Martino Grande, Romualdo Zanchetta, Nadia Gismondo, Maria Rita Mileto, Davide Mancon, Alessandro Gourlay, Louise Jane Vaccines (Basel) Article The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers. MDPI 2021-04-01 /pmc/articles/PMC8066017/ /pubmed/33915716 http://dx.doi.org/10.3390/vaccines9040322 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
De Benedetti, Stefano
Di Pisa, Flavio
Fassi, Enrico Mario Alessandro
Cretich, Marina
Musicò, Angelo
Frigerio, Roberto
Mussida, Alessandro
Bombaci, Mauro
Grifantini, Renata
Colombo, Giorgio
Bolognesi, Martino
Grande, Romualdo
Zanchetta, Nadia
Gismondo, Maria Rita
Mileto, Davide
Mancon, Alessandro
Gourlay, Louise Jane
Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title_full Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title_fullStr Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title_full_unstemmed Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title_short Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
title_sort structure, immunoreactivity, and in silico epitope determination of smspi s. mansoni serpin for immunodiagnostic application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066017/
https://www.ncbi.nlm.nih.gov/pubmed/33915716
http://dx.doi.org/10.3390/vaccines9040322
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