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Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections
Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviv...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066087/ https://www.ncbi.nlm.nih.gov/pubmed/33807442 http://dx.doi.org/10.3390/v13040596 |
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author | Stiasny, Karin Malafa, Stefan Aberle, Stephan W. Medits, Iris Tsouchnikas, Georgios Aberle, Judith H. Holzmann, Heidemarie Heinz, Franz X. |
author_facet | Stiasny, Karin Malafa, Stefan Aberle, Stephan W. Medits, Iris Tsouchnikas, Georgios Aberle, Judith H. Holzmann, Heidemarie Heinz, Franz X. |
author_sort | Stiasny, Karin |
collection | PubMed |
description | Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances. |
format | Online Article Text |
id | pubmed-8066087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80660872021-04-25 Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections Stiasny, Karin Malafa, Stefan Aberle, Stephan W. Medits, Iris Tsouchnikas, Georgios Aberle, Judith H. Holzmann, Heidemarie Heinz, Franz X. Viruses Article Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances. MDPI 2021-03-31 /pmc/articles/PMC8066087/ /pubmed/33807442 http://dx.doi.org/10.3390/v13040596 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stiasny, Karin Malafa, Stefan Aberle, Stephan W. Medits, Iris Tsouchnikas, Georgios Aberle, Judith H. Holzmann, Heidemarie Heinz, Franz X. Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title_full | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title_fullStr | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title_full_unstemmed | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title_short | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
title_sort | different cross-reactivities of igm responses in dengue, zika and tick-borne encephalitis virus infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066087/ https://www.ncbi.nlm.nih.gov/pubmed/33807442 http://dx.doi.org/10.3390/v13040596 |
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