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Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts

In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in P. falciparum (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive wit...

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Autores principales: Yadavalli, Raghavendra, Peterson, John W., Drazba, Judith A., Sam-Yellowe, Tobili Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066109/
https://www.ncbi.nlm.nih.gov/pubmed/33916455
http://dx.doi.org/10.3390/pathogens10040431
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author Yadavalli, Raghavendra
Peterson, John W.
Drazba, Judith A.
Sam-Yellowe, Tobili Y.
author_facet Yadavalli, Raghavendra
Peterson, John W.
Drazba, Judith A.
Sam-Yellowe, Tobili Y.
author_sort Yadavalli, Raghavendra
collection PubMed
description In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in P. falciparum (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive with PfMC-2TM. PfMC-2TM is sensitive to BFA treatment and permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associated with the MC and infected erythrocyte membrane and was resistant to extraction with alkaline sodium carbonate, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs.
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spelling pubmed-80661092021-04-25 Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts Yadavalli, Raghavendra Peterson, John W. Drazba, Judith A. Sam-Yellowe, Tobili Y. Pathogens Article In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in P. falciparum (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive with PfMC-2TM. PfMC-2TM is sensitive to BFA treatment and permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associated with the MC and infected erythrocyte membrane and was resistant to extraction with alkaline sodium carbonate, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs. MDPI 2021-04-05 /pmc/articles/PMC8066109/ /pubmed/33916455 http://dx.doi.org/10.3390/pathogens10040431 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yadavalli, Raghavendra
Peterson, John W.
Drazba, Judith A.
Sam-Yellowe, Tobili Y.
Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title_full Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title_fullStr Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title_full_unstemmed Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title_short Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts
title_sort trafficking and association of plasmodium falciparum mc-2tm with the maurer’s clefts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066109/
https://www.ncbi.nlm.nih.gov/pubmed/33916455
http://dx.doi.org/10.3390/pathogens10040431
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