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Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics

Timely diagnosis is crucial to improve the long-term survival of bladder cancer (BC) patients. The discovery of new BC biomarkers based in urine analysis is very attractive because this biofluid is in direct contact with the inner bladder layer, in which most of the neoplasms develop, and is non-inv...

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Autores principales: Pinto, Joana, Carapito, Ângela, Amaro, Filipa, Lima, Ana Rita, Carvalho-Maia, Carina, Martins, Maria Conceição, Jerónimo, Carmen, Henrique, Rui, Bastos, Maria de Lourdes, Guedes de Pinho, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066175/
https://www.ncbi.nlm.nih.gov/pubmed/33810601
http://dx.doi.org/10.3390/metabo11040199
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author Pinto, Joana
Carapito, Ângela
Amaro, Filipa
Lima, Ana Rita
Carvalho-Maia, Carina
Martins, Maria Conceição
Jerónimo, Carmen
Henrique, Rui
Bastos, Maria de Lourdes
Guedes de Pinho, Paula
author_facet Pinto, Joana
Carapito, Ângela
Amaro, Filipa
Lima, Ana Rita
Carvalho-Maia, Carina
Martins, Maria Conceição
Jerónimo, Carmen
Henrique, Rui
Bastos, Maria de Lourdes
Guedes de Pinho, Paula
author_sort Pinto, Joana
collection PubMed
description Timely diagnosis is crucial to improve the long-term survival of bladder cancer (BC) patients. The discovery of new BC biomarkers based in urine analysis is very attractive because this biofluid is in direct contact with the inner bladder layer, in which most of the neoplasms develop, and is non-invasively collected. Hence, this work aimed to unveil alterations in the urinary volatile profile of patients diagnosed with BC compared with cancer-free individuals, as well as differences among patients diagnosed at different tumor stages, to identify candidate biomarkers for non-invasive BC diagnosis and staging. Urine analysis was performed by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results unveiled that BC patients have a distinct urinary volatile profile characterized by higher levels of several alkanes and aromatic compounds, and lower levels of aldehydes, ketones and monoterpenes. Seventeen significantly altered volatiles were used to evaluate the performance for overall BC detection, disclosing 70% sensitivity, 89% specificity and 80% accuracy. Moreover, distinct urinary volatile profiles were found among patients diagnosed at different tumor stages (Ta/Tis, T1 and ≥T2). This work identified distinct urinary volatile signatures of BC patients with potential for non-invasive detection and staging of bladder cancer.
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spelling pubmed-80661752021-04-25 Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics Pinto, Joana Carapito, Ângela Amaro, Filipa Lima, Ana Rita Carvalho-Maia, Carina Martins, Maria Conceição Jerónimo, Carmen Henrique, Rui Bastos, Maria de Lourdes Guedes de Pinho, Paula Metabolites Article Timely diagnosis is crucial to improve the long-term survival of bladder cancer (BC) patients. The discovery of new BC biomarkers based in urine analysis is very attractive because this biofluid is in direct contact with the inner bladder layer, in which most of the neoplasms develop, and is non-invasively collected. Hence, this work aimed to unveil alterations in the urinary volatile profile of patients diagnosed with BC compared with cancer-free individuals, as well as differences among patients diagnosed at different tumor stages, to identify candidate biomarkers for non-invasive BC diagnosis and staging. Urine analysis was performed by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results unveiled that BC patients have a distinct urinary volatile profile characterized by higher levels of several alkanes and aromatic compounds, and lower levels of aldehydes, ketones and monoterpenes. Seventeen significantly altered volatiles were used to evaluate the performance for overall BC detection, disclosing 70% sensitivity, 89% specificity and 80% accuracy. Moreover, distinct urinary volatile profiles were found among patients diagnosed at different tumor stages (Ta/Tis, T1 and ≥T2). This work identified distinct urinary volatile signatures of BC patients with potential for non-invasive detection and staging of bladder cancer. MDPI 2021-03-26 /pmc/articles/PMC8066175/ /pubmed/33810601 http://dx.doi.org/10.3390/metabo11040199 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Pinto, Joana
Carapito, Ângela
Amaro, Filipa
Lima, Ana Rita
Carvalho-Maia, Carina
Martins, Maria Conceição
Jerónimo, Carmen
Henrique, Rui
Bastos, Maria de Lourdes
Guedes de Pinho, Paula
Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title_full Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title_fullStr Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title_full_unstemmed Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title_short Discovery of Volatile Biomarkers for Bladder Cancer Detection and Staging through Urine Metabolomics
title_sort discovery of volatile biomarkers for bladder cancer detection and staging through urine metabolomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066175/
https://www.ncbi.nlm.nih.gov/pubmed/33810601
http://dx.doi.org/10.3390/metabo11040199
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