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The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by elect...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066245/ https://www.ncbi.nlm.nih.gov/pubmed/33916638 http://dx.doi.org/10.3390/nano11040922 |
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author | Opálková Šišková, Alena Bučková, Mária Kroneková, Zuzana Kleinová, Angela Nagy, Štefan Rydz, Joanna Opálek, Andrej Sláviková, Monika Eckstein Andicsová, Anita |
author_facet | Opálková Šišková, Alena Bučková, Mária Kroneková, Zuzana Kleinová, Angela Nagy, Štefan Rydz, Joanna Opálek, Andrej Sláviková, Monika Eckstein Andicsová, Anita |
author_sort | Opálková Šišková, Alena |
collection | PubMed |
description | Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated. |
format | Online Article Text |
id | pubmed-8066245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80662452021-04-25 The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application Opálková Šišková, Alena Bučková, Mária Kroneková, Zuzana Kleinová, Angela Nagy, Štefan Rydz, Joanna Opálek, Andrej Sláviková, Monika Eckstein Andicsová, Anita Nanomaterials (Basel) Article Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated. MDPI 2021-04-04 /pmc/articles/PMC8066245/ /pubmed/33916638 http://dx.doi.org/10.3390/nano11040922 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Opálková Šišková, Alena Bučková, Mária Kroneková, Zuzana Kleinová, Angela Nagy, Štefan Rydz, Joanna Opálek, Andrej Sláviková, Monika Eckstein Andicsová, Anita The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title | The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title_full | The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title_fullStr | The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title_full_unstemmed | The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title_short | The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application |
title_sort | drug-loaded electrospun poly(ε-caprolactone) mats for therapeutic application |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066245/ https://www.ncbi.nlm.nih.gov/pubmed/33916638 http://dx.doi.org/10.3390/nano11040922 |
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