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The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by elect...

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Autores principales: Opálková Šišková, Alena, Bučková, Mária, Kroneková, Zuzana, Kleinová, Angela, Nagy, Štefan, Rydz, Joanna, Opálek, Andrej, Sláviková, Monika, Eckstein Andicsová, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066245/
https://www.ncbi.nlm.nih.gov/pubmed/33916638
http://dx.doi.org/10.3390/nano11040922
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author Opálková Šišková, Alena
Bučková, Mária
Kroneková, Zuzana
Kleinová, Angela
Nagy, Štefan
Rydz, Joanna
Opálek, Andrej
Sláviková, Monika
Eckstein Andicsová, Anita
author_facet Opálková Šišková, Alena
Bučková, Mária
Kroneková, Zuzana
Kleinová, Angela
Nagy, Štefan
Rydz, Joanna
Opálek, Andrej
Sláviková, Monika
Eckstein Andicsová, Anita
author_sort Opálková Šišková, Alena
collection PubMed
description Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated.
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spelling pubmed-80662452021-04-25 The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application Opálková Šišková, Alena Bučková, Mária Kroneková, Zuzana Kleinová, Angela Nagy, Štefan Rydz, Joanna Opálek, Andrej Sláviková, Monika Eckstein Andicsová, Anita Nanomaterials (Basel) Article Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated. MDPI 2021-04-04 /pmc/articles/PMC8066245/ /pubmed/33916638 http://dx.doi.org/10.3390/nano11040922 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Opálková Šišková, Alena
Bučková, Mária
Kroneková, Zuzana
Kleinová, Angela
Nagy, Štefan
Rydz, Joanna
Opálek, Andrej
Sláviková, Monika
Eckstein Andicsová, Anita
The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title_full The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title_fullStr The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title_full_unstemmed The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title_short The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application
title_sort drug-loaded electrospun poly(ε-caprolactone) mats for therapeutic application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066245/
https://www.ncbi.nlm.nih.gov/pubmed/33916638
http://dx.doi.org/10.3390/nano11040922
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