Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting

We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable o...

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Detalles Bibliográficos
Autores principales: Zhukov, Alex A., Pritchard, Robyn H., Withers, Mick J., Hailes, Tony, Gold, Richard D., Hayes, Calum, la Cour, Mette F., Hussein, Fred, Rogers, Salman Samson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066247/
https://www.ncbi.nlm.nih.gov/pubmed/33918161
http://dx.doi.org/10.3390/mi12040389
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author Zhukov, Alex A.
Pritchard, Robyn H.
Withers, Mick J.
Hailes, Tony
Gold, Richard D.
Hayes, Calum
la Cour, Mette F.
Hussein, Fred
Rogers, Salman Samson
author_facet Zhukov, Alex A.
Pritchard, Robyn H.
Withers, Mick J.
Hailes, Tony
Gold, Richard D.
Hayes, Calum
la Cour, Mette F.
Hussein, Fred
Rogers, Salman Samson
author_sort Zhukov, Alex A.
collection PubMed
description We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable of sorting lymphocyte-sized particles at 16 times the rate of our single-stream VACS devices, and approximately 10 times the rate of commercial cell sorters for an equivalent procedure. We believe this opens the potential to scale cell sorting for applications requiring the processing of much greater cell numbers than currently possible with conventional cell sorting.
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spelling pubmed-80662472021-04-25 Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting Zhukov, Alex A. Pritchard, Robyn H. Withers, Mick J. Hailes, Tony Gold, Richard D. Hayes, Calum la Cour, Mette F. Hussein, Fred Rogers, Salman Samson Micromachines (Basel) Communication We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable of sorting lymphocyte-sized particles at 16 times the rate of our single-stream VACS devices, and approximately 10 times the rate of commercial cell sorters for an equivalent procedure. We believe this opens the potential to scale cell sorting for applications requiring the processing of much greater cell numbers than currently possible with conventional cell sorting. MDPI 2021-04-02 /pmc/articles/PMC8066247/ /pubmed/33918161 http://dx.doi.org/10.3390/mi12040389 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Zhukov, Alex A.
Pritchard, Robyn H.
Withers, Mick J.
Hailes, Tony
Gold, Richard D.
Hayes, Calum
la Cour, Mette F.
Hussein, Fred
Rogers, Salman Samson
Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title_full Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title_fullStr Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title_full_unstemmed Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title_short Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
title_sort extremely high-throughput parallel microfluidic vortex-actuated cell sorting
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066247/
https://www.ncbi.nlm.nih.gov/pubmed/33918161
http://dx.doi.org/10.3390/mi12040389
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