Cargando…

Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia

Understanding the distribution, antimicrobial resistance (AMR), and risk factors associated with multidrug-resistant (MDR) and methicillin-resistant staphylococci (MRS) isolated from cats admitted to veterinary clinics may decrease the risk of MDR and MRS transmission to humans and other cats. As su...

Descripción completa

Detalles Bibliográficos
Autores principales: Elmoslemany, Ahmed, Elsohaby, Ibrahim, Alorabi, Mohammed, Alkafafy, Mohamed, Al-Marri, Theeb, Aldoweriej, Ali, Alaql, Fanan A., Almubarak, Abdullah, Fayez, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066541/
https://www.ncbi.nlm.nih.gov/pubmed/33807318
http://dx.doi.org/10.3390/antibiotics10040367
_version_ 1783682593128972288
author Elmoslemany, Ahmed
Elsohaby, Ibrahim
Alorabi, Mohammed
Alkafafy, Mohamed
Al-Marri, Theeb
Aldoweriej, Ali
Alaql, Fanan A.
Almubarak, Abdullah
Fayez, Mahmoud
author_facet Elmoslemany, Ahmed
Elsohaby, Ibrahim
Alorabi, Mohammed
Alkafafy, Mohamed
Al-Marri, Theeb
Aldoweriej, Ali
Alaql, Fanan A.
Almubarak, Abdullah
Fayez, Mahmoud
author_sort Elmoslemany, Ahmed
collection PubMed
description Understanding the distribution, antimicrobial resistance (AMR), and risk factors associated with multidrug-resistant (MDR) and methicillin-resistant staphylococci (MRS) isolated from cats admitted to veterinary clinics may decrease the risk of MDR and MRS transmission to humans and other cats. As such, the objectives of this study were to investigate the diversity in Staphylococcus spp. recovered from different anatomical locations in healthy and diseased cats and to determine the occurrence of MDR and MRS spp. as well as possible risk factors associated with colonization in these cats. Five swabs were collected from the anus, skin, ear canal, conjunctival sac, and nares of each cat (209 healthy and 191 diseased) admitted to a veterinary clinic in Eastern Province, Saudi Arabia, between January and December 2018. Prior to sample collection, cat owners completed a questionnaire collecting information on cat demographics, health status, management, and antimicrobial usage. In total, 179 Staphylococcus isolates were recovered from healthy (n = 71) and diseased (n = 108) cats, including 94 (52.5%) coagulase-positive staphylococci (CoPS), and 85 (47.5%) coagulase-negative staphylococci (CoNS). Five Staphylococcus spp. were identified, namely, Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus felis, Staphylococcus capitis, and Staphylococcus saprophyticus. Staphylococcus isolates were most commonly resistant to penicillin (56.4%) and ciprofloxacin (25.7%); however, no isolate was resistant to clindamycin. Thirty (16.8%) Staphylococcus spp. (24 S. aureus and 6 S. pseudintermedius) isolates were MDR, with resistance to up to six different antibiotic classes. Only 17 (9.5%) Staphylococcus spp. (15 methicillin-resistant S. aureus and 2 methicillin-resistant S. pseudintermedius) harbored the mecA gene. Risk factor analysis showed that cats with a history of antibiotic therapy, those raised mainly indoors with a child, and those who visit a veterinary clinic for treatment were at higher risk of MDR and MRS colonization. In conclusion, MDR and MRS were common in healthy and diseased cats in Saudi Arabia. Thus, an effective antimicrobial stewardship program and further studies using a One Health approach are required to investigate the role of cats as vectors for AMR transmission to humans.
format Online
Article
Text
id pubmed-8066541
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80665412021-04-25 Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia Elmoslemany, Ahmed Elsohaby, Ibrahim Alorabi, Mohammed Alkafafy, Mohamed Al-Marri, Theeb Aldoweriej, Ali Alaql, Fanan A. Almubarak, Abdullah Fayez, Mahmoud Antibiotics (Basel) Article Understanding the distribution, antimicrobial resistance (AMR), and risk factors associated with multidrug-resistant (MDR) and methicillin-resistant staphylococci (MRS) isolated from cats admitted to veterinary clinics may decrease the risk of MDR and MRS transmission to humans and other cats. As such, the objectives of this study were to investigate the diversity in Staphylococcus spp. recovered from different anatomical locations in healthy and diseased cats and to determine the occurrence of MDR and MRS spp. as well as possible risk factors associated with colonization in these cats. Five swabs were collected from the anus, skin, ear canal, conjunctival sac, and nares of each cat (209 healthy and 191 diseased) admitted to a veterinary clinic in Eastern Province, Saudi Arabia, between January and December 2018. Prior to sample collection, cat owners completed a questionnaire collecting information on cat demographics, health status, management, and antimicrobial usage. In total, 179 Staphylococcus isolates were recovered from healthy (n = 71) and diseased (n = 108) cats, including 94 (52.5%) coagulase-positive staphylococci (CoPS), and 85 (47.5%) coagulase-negative staphylococci (CoNS). Five Staphylococcus spp. were identified, namely, Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus felis, Staphylococcus capitis, and Staphylococcus saprophyticus. Staphylococcus isolates were most commonly resistant to penicillin (56.4%) and ciprofloxacin (25.7%); however, no isolate was resistant to clindamycin. Thirty (16.8%) Staphylococcus spp. (24 S. aureus and 6 S. pseudintermedius) isolates were MDR, with resistance to up to six different antibiotic classes. Only 17 (9.5%) Staphylococcus spp. (15 methicillin-resistant S. aureus and 2 methicillin-resistant S. pseudintermedius) harbored the mecA gene. Risk factor analysis showed that cats with a history of antibiotic therapy, those raised mainly indoors with a child, and those who visit a veterinary clinic for treatment were at higher risk of MDR and MRS colonization. In conclusion, MDR and MRS were common in healthy and diseased cats in Saudi Arabia. Thus, an effective antimicrobial stewardship program and further studies using a One Health approach are required to investigate the role of cats as vectors for AMR transmission to humans. MDPI 2021-03-31 /pmc/articles/PMC8066541/ /pubmed/33807318 http://dx.doi.org/10.3390/antibiotics10040367 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elmoslemany, Ahmed
Elsohaby, Ibrahim
Alorabi, Mohammed
Alkafafy, Mohamed
Al-Marri, Theeb
Aldoweriej, Ali
Alaql, Fanan A.
Almubarak, Abdullah
Fayez, Mahmoud
Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title_full Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title_fullStr Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title_full_unstemmed Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title_short Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia
title_sort diversity and risk factors associated with multidrug and methicillin-resistant staphylococci isolated from cats admitted to a veterinary clinic in eastern province, saudi arabia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066541/
https://www.ncbi.nlm.nih.gov/pubmed/33807318
http://dx.doi.org/10.3390/antibiotics10040367
work_keys_str_mv AT elmoslemanyahmed diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT elsohabyibrahim diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT alorabimohammed diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT alkafafymohamed diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT almarritheeb diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT aldoweriejali diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT alaqlfanana diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT almubarakabdullah diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia
AT fayezmahmoud diversityandriskfactorsassociatedwithmultidrugandmethicillinresistantstaphylococciisolatedfromcatsadmittedtoaveterinaryclinicineasternprovincesaudiarabia