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Embedded Immunodetection System for Fecal Occult Blood

In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip...

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Detalles Bibliográficos
Autores principales: Lin, Kai-Wen, Chang, Yu-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066604/
https://www.ncbi.nlm.nih.gov/pubmed/33916834
http://dx.doi.org/10.3390/bios11040106
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author Lin, Kai-Wen
Chang, Yu-Chi
author_facet Lin, Kai-Wen
Chang, Yu-Chi
author_sort Lin, Kai-Wen
collection PubMed
description In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python include the main program, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing. After verification of each part of the system, it was found that the rapid test system successfully detects from 0 ng/mL to 400 ng/mL of FOB with coefficients of variation (CV) below 3.7% and 1000 ng/mL with a CV only at 7.41%.
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spelling pubmed-80666042021-04-25 Embedded Immunodetection System for Fecal Occult Blood Lin, Kai-Wen Chang, Yu-Chi Biosensors (Basel) Communication In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python include the main program, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing. After verification of each part of the system, it was found that the rapid test system successfully detects from 0 ng/mL to 400 ng/mL of FOB with coefficients of variation (CV) below 3.7% and 1000 ng/mL with a CV only at 7.41%. MDPI 2021-04-03 /pmc/articles/PMC8066604/ /pubmed/33916834 http://dx.doi.org/10.3390/bios11040106 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lin, Kai-Wen
Chang, Yu-Chi
Embedded Immunodetection System for Fecal Occult Blood
title Embedded Immunodetection System for Fecal Occult Blood
title_full Embedded Immunodetection System for Fecal Occult Blood
title_fullStr Embedded Immunodetection System for Fecal Occult Blood
title_full_unstemmed Embedded Immunodetection System for Fecal Occult Blood
title_short Embedded Immunodetection System for Fecal Occult Blood
title_sort embedded immunodetection system for fecal occult blood
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066604/
https://www.ncbi.nlm.nih.gov/pubmed/33916834
http://dx.doi.org/10.3390/bios11040106
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