Cargando…

Adjusting the Cut-Off and Maximum Pool Size in RT-qPCR Pool Testing for SARS-CoV-2

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-CoV-2 RNA is an essential test to monitor the occurrence of COVID-19. A methodology is proposed for the determination of maximum pool size and adjustments of cut-off values of cycle threshold ([Formula: s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Costa, Murilo S., Sato, Hugo I., Rocha, Raissa P., Carvalho, Alex F., Guimarães, Nathalia S., Machado, Elaine L., Alves, Claudia R. L., Teixeira, Santuza M. R., Takahashi, Ricardo H. C., Tupinambás, Unaí, da Fonseca, Flávio G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066647/ https://www.ncbi.nlm.nih.gov/pubmed/33810324 http://dx.doi.org/10.3390/v13040557

Ejemplares similares

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of SARS-CoV-2 through pool testing for COVID-19: an integrative review
por: Costa, Murilo Soares, et al.
Publicado: (2021)

Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling
por: Alcoba-Florez, Julia, et al.
Publicado: (2021)

Improvement of SARS-COV-2 screening using pooled sampling testing in limited RT-qPCR resources
por: Bensaada, Mostefa, et al.
Publicado: (2022)

Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
por: Volpato, Fabiana, et al.
Publicado: (2020)

Pooling RT-qPCR testing for SARS-CoV-2 in 1000 individuals of healthy and infection-suspected patients
por: Hirotsu, Yosuke, et al.
Publicado: (2020)

Pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools - a cluster randomised trial
por: Joachim, Alexander, et al.
Publicado: (2021)

Flock sensitivity and specificity of pooled fecal qPCR and pooled serum ELISA for screening ovine paratuberculosis
por: Mathevon, Yoann, et al.
Publicado: (2019)

Implementing the Lolli-Method and pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools: a pilot project
por: Kretschmer, Alina Chloé, et al.
Publicado: (2022)

Accelerating the Laboratory Testing Capacity through Saliva Pooling Prior to Direct RT-qPCR for SARS-CoV-2 Detection
por: Kaisar, Maria Mardalena Martini, et al.
Publicado: (2022)

Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
por: Muniesa, Ana, et al.
Publicado: (2014)

Model-based prediction of maximum pool size in the ribbon synapse
por: Parmelee, Caitlyn M, et al.
Publicado: (2015)

Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution
por: Christoff, Ana Paula, et al.
Publicado: (2021)

RT-qPCR-based tests for SARS-CoV-2 detection in pooled saliva samples for massive population screening to monitor epidemics
por: Różański, Michał, et al.
Publicado: (2022)

Maximum Likelihood Estimation of Frequencies of Known Haplotypes from Pooled Sequence Data
por: Kessner, Darren, et al.
Publicado: (2013)

"Sample pooling of RNA extracts to speed up SARS-CoV-2 diagnosis using CDC FDA EUA RT-qPCR kit"
por: Freire-Paspuel, Byron, et al.
Publicado: (2020)

Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes
por: Verwilt, Jasper, et al.
Publicado: (2022)

Integrating Magnetic-Bead-Based Sample Extraction and Molecular Barcoding for the One-Step Pooled RT-qPCR Assay of Viral Pathogens without Retesting
por: Zhuang, Xinyu, et al.
Publicado: (2023)

siPools: highly complex but accurately defined siRNA pools eliminate off-target effects
por: Hannus, Michael, et al.
Publicado: (2014)

Plan to pool bird-flu data takes off
por: Pearson, Helen
Publicado: (2006)

Sample pooling for SARS-CoV-2 RT-PCR screening
por: de Salazar, Adolfo, et al.
Publicado: (2020)

Pooling skin swabs does not inhibit qPCR detection of amphibian chytrid infection
por: Sabino-Pinto, Joana, et al.
Publicado: (2019)

Pooling Data on Pools: Genotoxicity of Chemicals in Indoor Swimming Pools
por: Spivey, Angela
Publicado: (2010)

SARS-CoV-2 RT-qPCR testing of pooled saliva samples: A case study of 824 asymptomatic individuals and a questionnaire survey in Japan
por: Oba, Junna, et al.
Publicado: (2022)

Pooled testing for COVID-19 diagnosis by real-time RT-PCR: A multi-site comparative evaluation of 5- & 10-sample pooling
por: Praharaj, Ira, et al.
Publicado: (2020)

Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
por: Petrovan, Vlad, et al.
Publicado: (2020)

Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis
por: Jaeger, Lauren Hubert, et al.
Publicado: (2021)

Evaluation of SARS CoV-2 RT-PCR in a multiple sample pool
por: Garg, Jaya, et al.
Publicado: (2021)

An accurate model for SARS-CoV-2 pooled RT-PCR test errors
por: Daon, Yair, et al.
Publicado: (2021)

A Compressed Sensing Approach to Pooled RT-PCR Testing for COVID-19 Detection
Publicado: (2021)

Evaluation of commercial RT-PCR kits for pooled clinical specimens in COVID-19 patients.
por: Arya, Akshay Kumar, et al.
Publicado: (2021)

To adjust or not to adjust: Cut-off scores in performance validity testing in Portuguese older adults with dementia
por: Fernandes, Sandra, et al.
Publicado: (2022)

SARS-CoV-2 surveillance by RT-qPCR-based pool testing of saliva swabs (lollipop method) at primary and special schools—A pilot study on feasibility and acceptability
por: Kästner, Anika, et al.
Publicado: (2022)

PCR effects of melting temperature adjustment of individual primers in degenerate primer pools
por: Naqib, Ankur, et al.
Publicado: (2019)

Hyperspectral Remote Sensing Image Classification Based on Maximum Overlap Pooling Convolutional Neural Network
por: Li, Chenming, et al.
Publicado: (2018)

Diagnostic performance of RT-PCR-based sample pooling strategy for the detection of SARS-CoV-2
por: Hueda-Zavaleta, Miguel, et al.
Publicado: (2022)

Effect of swab pooling on the Accula point-of-care RT-PCR for SARS-CoV-2 detection
por: Lancelot, Moira, et al.
Publicado: (2023)

A pooled RT-PCR testing strategy for more efficient COVID-19 pandemic management
por: Ayaz, Akif, et al.
Publicado: (2022)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

The hippopotamus pool /
por: Peters, Elizabeth
Publicado: (1996)

Cannot write session to /tmp/vufind_sessions/sess_5jvuv943fdr7316749n2n3csme