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Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens

BACKGROUND: Bacteroides fragilis is a part of the normal gastrointestinal flora, but it is also the most common anaerobic bacteria causing the infection. It is highly resistant to antibiotics and contains abundant antibiotic resistance mechanisms. METHODS: The antibiotic resistance pattern of 78 iso...

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Autores principales: Jasemi, Seyedesomaye, Emaneini, Mohammad, Ahmadinejad, Zahra, Fazeli, Mohammad Sadegh, Sechi, Leonardo A., Sadeghpour Heravi, Fatemah, Feizabadi, Mohammad Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066845/
https://www.ncbi.nlm.nih.gov/pubmed/33892721
http://dx.doi.org/10.1186/s12941-021-00435-w
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author Jasemi, Seyedesomaye
Emaneini, Mohammad
Ahmadinejad, Zahra
Fazeli, Mohammad Sadegh
Sechi, Leonardo A.
Sadeghpour Heravi, Fatemah
Feizabadi, Mohammad Mehdi
author_facet Jasemi, Seyedesomaye
Emaneini, Mohammad
Ahmadinejad, Zahra
Fazeli, Mohammad Sadegh
Sechi, Leonardo A.
Sadeghpour Heravi, Fatemah
Feizabadi, Mohammad Mehdi
author_sort Jasemi, Seyedesomaye
collection PubMed
description BACKGROUND: Bacteroides fragilis is a part of the normal gastrointestinal flora, but it is also the most common anaerobic bacteria causing the infection. It is highly resistant to antibiotics and contains abundant antibiotic resistance mechanisms. METHODS: The antibiotic resistance pattern of 78 isolates of B. fragilis (22 strains from clinical samples and 56 strains from the colorectal tissue) was investigated using agar dilution method. The gene encoding Bacteroides fargilis toxin bft, and antibiotic resistance genes were targeted by PCR assay. RESULTS: The highest rate of resistance was observed for penicillin G (100%) followed by tetracycline (74.4%), clindamycin (41%) and cefoxitin (38.5%). Only a single isolate showed resistance to imipenem which contained cfiA and IS1186 genes. All isolates were susceptible to metronidazole. Accordingly, tetQ (87.2%), cepA (73.1%) and ermF (64.1%) were the most abundant antibiotic-resistant genes identified in this study. MIC values for penicillin, cefoxitin and clindamycin were significantly different among isolates with the cepA, cfxA and ermF in compare with those lacking such genes. In addition, 22.7 and 17.8% of clinical and GIT isolates had the bft gene, respectively. CONCLUSIONS: The finding of this study shows that metronidazole is highly in vitro active agent against all of B. fragilis isolates and remain the first-line antimicrobial for empirical therapy.
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spelling pubmed-80668452021-04-26 Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens Jasemi, Seyedesomaye Emaneini, Mohammad Ahmadinejad, Zahra Fazeli, Mohammad Sadegh Sechi, Leonardo A. Sadeghpour Heravi, Fatemah Feizabadi, Mohammad Mehdi Ann Clin Microbiol Antimicrob Research BACKGROUND: Bacteroides fragilis is a part of the normal gastrointestinal flora, but it is also the most common anaerobic bacteria causing the infection. It is highly resistant to antibiotics and contains abundant antibiotic resistance mechanisms. METHODS: The antibiotic resistance pattern of 78 isolates of B. fragilis (22 strains from clinical samples and 56 strains from the colorectal tissue) was investigated using agar dilution method. The gene encoding Bacteroides fargilis toxin bft, and antibiotic resistance genes were targeted by PCR assay. RESULTS: The highest rate of resistance was observed for penicillin G (100%) followed by tetracycline (74.4%), clindamycin (41%) and cefoxitin (38.5%). Only a single isolate showed resistance to imipenem which contained cfiA and IS1186 genes. All isolates were susceptible to metronidazole. Accordingly, tetQ (87.2%), cepA (73.1%) and ermF (64.1%) were the most abundant antibiotic-resistant genes identified in this study. MIC values for penicillin, cefoxitin and clindamycin were significantly different among isolates with the cepA, cfxA and ermF in compare with those lacking such genes. In addition, 22.7 and 17.8% of clinical and GIT isolates had the bft gene, respectively. CONCLUSIONS: The finding of this study shows that metronidazole is highly in vitro active agent against all of B. fragilis isolates and remain the first-line antimicrobial for empirical therapy. BioMed Central 2021-04-23 /pmc/articles/PMC8066845/ /pubmed/33892721 http://dx.doi.org/10.1186/s12941-021-00435-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jasemi, Seyedesomaye
Emaneini, Mohammad
Ahmadinejad, Zahra
Fazeli, Mohammad Sadegh
Sechi, Leonardo A.
Sadeghpour Heravi, Fatemah
Feizabadi, Mohammad Mehdi
Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title_full Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title_fullStr Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title_full_unstemmed Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title_short Antibiotic resistance pattern of Bacteroides fragilis isolated from clinical and colorectal specimens
title_sort antibiotic resistance pattern of bacteroides fragilis isolated from clinical and colorectal specimens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066845/
https://www.ncbi.nlm.nih.gov/pubmed/33892721
http://dx.doi.org/10.1186/s12941-021-00435-w
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