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The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field
Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066883/ https://www.ncbi.nlm.nih.gov/pubmed/33916308 http://dx.doi.org/10.3390/v13040606 |
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author | Wang, Lidong Zhang, Yanyan Chen, Teng Mi, Lijuan Sun, Xuefei Zhou, Xintao Miao, Faming Zhang, Shoufeng Liu, Ye Hu, Rongliang |
author_facet | Wang, Lidong Zhang, Yanyan Chen, Teng Mi, Lijuan Sun, Xuefei Zhou, Xintao Miao, Faming Zhang, Shoufeng Liu, Ye Hu, Rongliang |
author_sort | Wang, Lidong |
collection | PubMed |
description | Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressing the capsid protein was constructed using the baculovirus expression vector system (BEVS). A subunit vaccine was developed based on the capsid protein with appropriate adjuvant. Then, a field trial was carried out in two districts in order to evaluate the efficiency of the subunit vaccine. The field trial indicated that in total, only 1.8% of the minks developed typical diarrhea in the vaccinated group compared with 74.5% in the control group. The vaccination could significantly reduce the infection rate of MiCV among the mink herds and could restrain the virus’ shedding from feces. Furthermore, the vaccinated group had a higher average litter size in the following year compared to the control group. Collectively, the results indicated that the subunit vaccine based on the capsid protein can provide reliable protection against MiCV infection. |
format | Online Article Text |
id | pubmed-8066883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80668832021-04-25 The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field Wang, Lidong Zhang, Yanyan Chen, Teng Mi, Lijuan Sun, Xuefei Zhou, Xintao Miao, Faming Zhang, Shoufeng Liu, Ye Hu, Rongliang Viruses Article Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressing the capsid protein was constructed using the baculovirus expression vector system (BEVS). A subunit vaccine was developed based on the capsid protein with appropriate adjuvant. Then, a field trial was carried out in two districts in order to evaluate the efficiency of the subunit vaccine. The field trial indicated that in total, only 1.8% of the minks developed typical diarrhea in the vaccinated group compared with 74.5% in the control group. The vaccination could significantly reduce the infection rate of MiCV among the mink herds and could restrain the virus’ shedding from feces. Furthermore, the vaccinated group had a higher average litter size in the following year compared to the control group. Collectively, the results indicated that the subunit vaccine based on the capsid protein can provide reliable protection against MiCV infection. MDPI 2021-04-01 /pmc/articles/PMC8066883/ /pubmed/33916308 http://dx.doi.org/10.3390/v13040606 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Lidong Zhang, Yanyan Chen, Teng Mi, Lijuan Sun, Xuefei Zhou, Xintao Miao, Faming Zhang, Shoufeng Liu, Ye Hu, Rongliang The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title | The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title_full | The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title_fullStr | The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title_full_unstemmed | The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title_short | The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field |
title_sort | mink circovirus capsid subunit expressed by recombinant baculovirus protects minks against refractory diarrhea in field |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066883/ https://www.ncbi.nlm.nih.gov/pubmed/33916308 http://dx.doi.org/10.3390/v13040606 |
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