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Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice

Rice is the most aluminum (Al)-tolerant species among the small grain cereals, but there are great variations in the Al tolerance between subspecies, with higher tolerance in japonica subspecies than indica subspecies. Here, we performed a screening of Al tolerance using 65 indica cultivars and foun...

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Autores principales: Sun, Li Ming, Che, Jing, Ma, Jian Feng, Shen, Ren Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066893/
https://www.ncbi.nlm.nih.gov/pubmed/33810593
http://dx.doi.org/10.3390/plants10040634
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author Sun, Li Ming
Che, Jing
Ma, Jian Feng
Shen, Ren Fang
author_facet Sun, Li Ming
Che, Jing
Ma, Jian Feng
Shen, Ren Fang
author_sort Sun, Li Ming
collection PubMed
description Rice is the most aluminum (Al)-tolerant species among the small grain cereals, but there are great variations in the Al tolerance between subspecies, with higher tolerance in japonica subspecies than indica subspecies. Here, we performed a screening of Al tolerance using 65 indica cultivars and found that there was also a large genotypic difference in Al tolerance among indica subspecies. Further characterization of two cultivars contrasting in Al tolerance showed that the expression level of ART1 (ALUMINUM RESISTANCE TRANSCRIPTION FACTOR 1) encoding a C2H2-type Zn-finger transcription factor, was higher in an Al-tolerant indica cultivar, Jinguoyin, than in an Al-sensitive indica cultivar, Kasalath. Furthermore, a dose-response experiment showed that ART1 expression was not induced by Al in both cultivars, but Jinguoyin always showed 5.9 to 11.4-fold higher expression compared with Kasalath, irrespectively of Al concentrations. Among genes regulated by ART1, 19 genes showed higher expression in Jinguoyin than in Kasalath. This is associated with less Al accumulation in the root tip cell wall in Jinguoyin. Sequence comparison of the 2-kb promoter region of ART1 revealed the extensive sequence polymorphism between two cultivars. Whole transcriptome analysis with RNA-seq revealed that more genes were up- and downregulated by Al in Kasalath than in Jinguoyin. Taken together, our results suggest that there is a large genotypic variation in Al tolerance in indica rice and that the different expression level of ART1 is responsible for the genotypic difference in the Al tolerance.
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spelling pubmed-80668932021-04-25 Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice Sun, Li Ming Che, Jing Ma, Jian Feng Shen, Ren Fang Plants (Basel) Article Rice is the most aluminum (Al)-tolerant species among the small grain cereals, but there are great variations in the Al tolerance between subspecies, with higher tolerance in japonica subspecies than indica subspecies. Here, we performed a screening of Al tolerance using 65 indica cultivars and found that there was also a large genotypic difference in Al tolerance among indica subspecies. Further characterization of two cultivars contrasting in Al tolerance showed that the expression level of ART1 (ALUMINUM RESISTANCE TRANSCRIPTION FACTOR 1) encoding a C2H2-type Zn-finger transcription factor, was higher in an Al-tolerant indica cultivar, Jinguoyin, than in an Al-sensitive indica cultivar, Kasalath. Furthermore, a dose-response experiment showed that ART1 expression was not induced by Al in both cultivars, but Jinguoyin always showed 5.9 to 11.4-fold higher expression compared with Kasalath, irrespectively of Al concentrations. Among genes regulated by ART1, 19 genes showed higher expression in Jinguoyin than in Kasalath. This is associated with less Al accumulation in the root tip cell wall in Jinguoyin. Sequence comparison of the 2-kb promoter region of ART1 revealed the extensive sequence polymorphism between two cultivars. Whole transcriptome analysis with RNA-seq revealed that more genes were up- and downregulated by Al in Kasalath than in Jinguoyin. Taken together, our results suggest that there is a large genotypic variation in Al tolerance in indica rice and that the different expression level of ART1 is responsible for the genotypic difference in the Al tolerance. MDPI 2021-03-26 /pmc/articles/PMC8066893/ /pubmed/33810593 http://dx.doi.org/10.3390/plants10040634 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Sun, Li Ming
Che, Jing
Ma, Jian Feng
Shen, Ren Fang
Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title_full Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title_fullStr Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title_full_unstemmed Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title_short Expression Level of Transcription Factor ART1 Is Responsible for Differential Aluminum Tolerance in Indica Rice
title_sort expression level of transcription factor art1 is responsible for differential aluminum tolerance in indica rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066893/
https://www.ncbi.nlm.nih.gov/pubmed/33810593
http://dx.doi.org/10.3390/plants10040634
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