A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing

An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antig...

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Autores principales: Cho, Seungchan, Allison, Jon C., Park, Kkothanahreum, No, Jin Sun, Lee, Seung-Ho, Park, Kyungmin, Kim, Jongwoo, Klein, Terry A., Kim, Heung-Chul, Kim, Won-Keun, Song, Jin-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067027/
https://www.ncbi.nlm.nih.gov/pubmed/33918345
http://dx.doi.org/10.3390/pathogens10040424
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author Cho, Seungchan
Allison, Jon C.
Park, Kkothanahreum
No, Jin Sun
Lee, Seung-Ho
Park, Kyungmin
Kim, Jongwoo
Klein, Terry A.
Kim, Heung-Chul
Kim, Won-Keun
Song, Jin-Won
author_facet Cho, Seungchan
Allison, Jon C.
Park, Kkothanahreum
No, Jin Sun
Lee, Seung-Ho
Park, Kyungmin
Kim, Jongwoo
Klein, Terry A.
Kim, Heung-Chul
Kim, Won-Keun
Song, Jin-Won
author_sort Cho, Seungchan
collection PubMed
description An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.
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spelling pubmed-80670272021-04-25 A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing Cho, Seungchan Allison, Jon C. Park, Kkothanahreum No, Jin Sun Lee, Seung-Ho Park, Kyungmin Kim, Jongwoo Klein, Terry A. Kim, Heung-Chul Kim, Won-Keun Song, Jin-Won Pathogens Brief Report An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain. MDPI 2021-04-02 /pmc/articles/PMC8067027/ /pubmed/33918345 http://dx.doi.org/10.3390/pathogens10040424 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Cho, Seungchan
Allison, Jon C.
Park, Kkothanahreum
No, Jin Sun
Lee, Seung-Ho
Park, Kyungmin
Kim, Jongwoo
Klein, Terry A.
Kim, Heung-Chul
Kim, Won-Keun
Song, Jin-Won
A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title_full A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title_fullStr A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title_full_unstemmed A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title_short A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
title_sort clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067027/
https://www.ncbi.nlm.nih.gov/pubmed/33918345
http://dx.doi.org/10.3390/pathogens10040424
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