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Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

SIMPLE SUMMARY: Chicken infectious anemia virus (CIAV) is an immunosuppressive pathogen of chickens. In the present study field, vaccine-derived CIAV strains were reported to circulate in different types of chicken flocks. Viruses were successfully obtained from non-invasive samples such as feathers...

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Autores principales: Quaglia, Giulia, Mescolini, Giulia, Catelli, Elena, Berto, Giacomo, Muccioli, Filippo, Lupini, Caterina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067058/
https://www.ncbi.nlm.nih.gov/pubmed/33801597
http://dx.doi.org/10.3390/ani11040944
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author Quaglia, Giulia
Mescolini, Giulia
Catelli, Elena
Berto, Giacomo
Muccioli, Filippo
Lupini, Caterina
author_facet Quaglia, Giulia
Mescolini, Giulia
Catelli, Elena
Berto, Giacomo
Muccioli, Filippo
Lupini, Caterina
author_sort Quaglia, Giulia
collection PubMed
description SIMPLE SUMMARY: Chicken infectious anemia virus (CIAV) is an immunosuppressive pathogen of chickens. In the present study field, vaccine-derived CIAV strains were reported to circulate in different types of chicken flocks. Viruses were successfully obtained from non-invasive samples such as feathers and environmental dust. Genome analysis showed that strains had heterogeneous sequences clustered into different genogroups that possessed genetic markers reported to be correlated with CIAV virulence. This survey contributes to the knowledge of field CIAV distribution maps and increases the existing information available on native isolates around the world. ABSTRACT: Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.
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spelling pubmed-80670582021-04-25 Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl Quaglia, Giulia Mescolini, Giulia Catelli, Elena Berto, Giacomo Muccioli, Filippo Lupini, Caterina Animals (Basel) Article SIMPLE SUMMARY: Chicken infectious anemia virus (CIAV) is an immunosuppressive pathogen of chickens. In the present study field, vaccine-derived CIAV strains were reported to circulate in different types of chicken flocks. Viruses were successfully obtained from non-invasive samples such as feathers and environmental dust. Genome analysis showed that strains had heterogeneous sequences clustered into different genogroups that possessed genetic markers reported to be correlated with CIAV virulence. This survey contributes to the knowledge of field CIAV distribution maps and increases the existing information available on native isolates around the world. ABSTRACT: Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide. MDPI 2021-03-27 /pmc/articles/PMC8067058/ /pubmed/33801597 http://dx.doi.org/10.3390/ani11040944 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Quaglia, Giulia
Mescolini, Giulia
Catelli, Elena
Berto, Giacomo
Muccioli, Filippo
Lupini, Caterina
Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title_full Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title_fullStr Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title_full_unstemmed Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title_short Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl
title_sort genetic heterogeneity among chicken infectious anemia viruses detected in italian fowl
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067058/
https://www.ncbi.nlm.nih.gov/pubmed/33801597
http://dx.doi.org/10.3390/ani11040944
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