SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix

Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of coval...

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Autores principales: Rippa, Massimo, Castagna, Riccardo, Sagnelli, Domenico, Vestri, Ambra, Borriello, Giorgia, Fusco, Giovanna, Zhou, Jun, Petti, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067257/
https://www.ncbi.nlm.nih.gov/pubmed/33807185
http://dx.doi.org/10.3390/nano11040886
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author Rippa, Massimo
Castagna, Riccardo
Sagnelli, Domenico
Vestri, Ambra
Borriello, Giorgia
Fusco, Giovanna
Zhou, Jun
Petti, Lucia
author_facet Rippa, Massimo
Castagna, Riccardo
Sagnelli, Domenico
Vestri, Ambra
Borriello, Giorgia
Fusco, Giovanna
Zhou, Jun
Petti, Lucia
author_sort Rippa, Massimo
collection PubMed
description Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (10(4) cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.
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spelling pubmed-80672572021-04-25 SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix Rippa, Massimo Castagna, Riccardo Sagnelli, Domenico Vestri, Ambra Borriello, Giorgia Fusco, Giovanna Zhou, Jun Petti, Lucia Nanomaterials (Basel) Communication Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (10(4) cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection. MDPI 2021-03-31 /pmc/articles/PMC8067257/ /pubmed/33807185 http://dx.doi.org/10.3390/nano11040886 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Rippa, Massimo
Castagna, Riccardo
Sagnelli, Domenico
Vestri, Ambra
Borriello, Giorgia
Fusco, Giovanna
Zhou, Jun
Petti, Lucia
SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title_full SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title_fullStr SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title_full_unstemmed SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title_short SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix
title_sort sers biosensor based on engineered 2d-aperiodic nanostructure for in-situ detection of viable brucella bacterium in complex matrix
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067257/
https://www.ncbi.nlm.nih.gov/pubmed/33807185
http://dx.doi.org/10.3390/nano11040886
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