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Spontaneous Abortion and Chikungunya Infection: Pathological Findings

Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of preg...

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Autores principales: Salomão, Natália, Brendolin, Michelle, Rabelo, Kíssila, Wakimoto, Mayumi, de Filippis, Ana Maria, dos Santos, Flavia, Moreira, Maria Elizabeth, Basílio-de-Oliveira, Carlos Alberto, Avvad-Portari, Elyzabeth, Paes, Marciano, Brasil, Patrícia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067258/
https://www.ncbi.nlm.nih.gov/pubmed/33806252
http://dx.doi.org/10.3390/v13040554
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author Salomão, Natália
Brendolin, Michelle
Rabelo, Kíssila
Wakimoto, Mayumi
de Filippis, Ana Maria
dos Santos, Flavia
Moreira, Maria Elizabeth
Basílio-de-Oliveira, Carlos Alberto
Avvad-Portari, Elyzabeth
Paes, Marciano
Brasil, Patrícia
author_facet Salomão, Natália
Brendolin, Michelle
Rabelo, Kíssila
Wakimoto, Mayumi
de Filippis, Ana Maria
dos Santos, Flavia
Moreira, Maria Elizabeth
Basílio-de-Oliveira, Carlos Alberto
Avvad-Portari, Elyzabeth
Paes, Marciano
Brasil, Patrícia
author_sort Salomão, Natália
collection PubMed
description Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death.
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spelling pubmed-80672582021-04-25 Spontaneous Abortion and Chikungunya Infection: Pathological Findings Salomão, Natália Brendolin, Michelle Rabelo, Kíssila Wakimoto, Mayumi de Filippis, Ana Maria dos Santos, Flavia Moreira, Maria Elizabeth Basílio-de-Oliveira, Carlos Alberto Avvad-Portari, Elyzabeth Paes, Marciano Brasil, Patrícia Viruses Article Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death. MDPI 2021-03-25 /pmc/articles/PMC8067258/ /pubmed/33806252 http://dx.doi.org/10.3390/v13040554 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Salomão, Natália
Brendolin, Michelle
Rabelo, Kíssila
Wakimoto, Mayumi
de Filippis, Ana Maria
dos Santos, Flavia
Moreira, Maria Elizabeth
Basílio-de-Oliveira, Carlos Alberto
Avvad-Portari, Elyzabeth
Paes, Marciano
Brasil, Patrícia
Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title_full Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title_fullStr Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title_full_unstemmed Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title_short Spontaneous Abortion and Chikungunya Infection: Pathological Findings
title_sort spontaneous abortion and chikungunya infection: pathological findings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067258/
https://www.ncbi.nlm.nih.gov/pubmed/33806252
http://dx.doi.org/10.3390/v13040554
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