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Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan

Middle East respiratory syndrome-related coronavirus (MERS-CoV) is a persistent zoonotic pathogen with frequent spillover from dromedary camels to humans in the Arabian Peninsula, resulting in limited outbreaks of MERS with a high case-fatality rate. Full genome sequence data from camel-derived MERS...

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Autores principales: Seifert, Stephanie N., Schulz, Jonathan E., Ricklefs, Stacy, Letko, Michael, Yabba, Elangeni, Hijazeen, Zaidoun S., Holloway, Peter, Al-Omari, Bilal, Talafha, Hani A., Tibbo, Markos, Adney, Danielle R., Guitian, Javier, Amarin, Nadim, Richt, Juergen A., McDowell, Chester, Steel, John, Abu-Basha, Ehab A., Al-Majali, Ahmad M., van Doremalen, Neeltje, Munster, Vincent J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067259/
https://www.ncbi.nlm.nih.gov/pubmed/33807288
http://dx.doi.org/10.3390/v13040592
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author Seifert, Stephanie N.
Schulz, Jonathan E.
Ricklefs, Stacy
Letko, Michael
Yabba, Elangeni
Hijazeen, Zaidoun S.
Holloway, Peter
Al-Omari, Bilal
Talafha, Hani A.
Tibbo, Markos
Adney, Danielle R.
Guitian, Javier
Amarin, Nadim
Richt, Juergen A.
McDowell, Chester
Steel, John
Abu-Basha, Ehab A.
Al-Majali, Ahmad M.
van Doremalen, Neeltje
Munster, Vincent J.
author_facet Seifert, Stephanie N.
Schulz, Jonathan E.
Ricklefs, Stacy
Letko, Michael
Yabba, Elangeni
Hijazeen, Zaidoun S.
Holloway, Peter
Al-Omari, Bilal
Talafha, Hani A.
Tibbo, Markos
Adney, Danielle R.
Guitian, Javier
Amarin, Nadim
Richt, Juergen A.
McDowell, Chester
Steel, John
Abu-Basha, Ehab A.
Al-Majali, Ahmad M.
van Doremalen, Neeltje
Munster, Vincent J.
author_sort Seifert, Stephanie N.
collection PubMed
description Middle East respiratory syndrome-related coronavirus (MERS-CoV) is a persistent zoonotic pathogen with frequent spillover from dromedary camels to humans in the Arabian Peninsula, resulting in limited outbreaks of MERS with a high case-fatality rate. Full genome sequence data from camel-derived MERS-CoV variants show diverse lineages circulating in domestic camels with frequent recombination. More than 90% of the available full MERS-CoV genome sequences derived from camels are from just two countries, the Kingdom of Saudi Arabia (KSA) and United Arab Emirates (UAE). In this study, we employ a novel method to amplify and sequence the partial MERS-CoV genome with high sensitivity from nasal swabs of infected camels. We recovered more than 99% of the MERS-CoV genome from field-collected samples with greater than 500 TCID(50) equivalent per nasal swab from camel herds sampled in Jordan in May 2016. Our subsequent analyses of 14 camel-derived MERS-CoV genomes show a striking lack of genetic diversity circulating in Jordan camels relative to MERS-CoV genome sequences derived from large camel markets in KSA and UAE. The low genetic diversity detected in Jordan camels during our study is consistent with a lack of endemic circulation in these camel herds and reflective of data from MERS outbreaks in humans dominated by nosocomial transmission following a single introduction as reported during the 2015 MERS outbreak in South Korea. Our data suggest transmission of MERS-CoV among two camel herds in Jordan in 2016 following a single introduction event.
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spelling pubmed-80672592021-04-25 Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan Seifert, Stephanie N. Schulz, Jonathan E. Ricklefs, Stacy Letko, Michael Yabba, Elangeni Hijazeen, Zaidoun S. Holloway, Peter Al-Omari, Bilal Talafha, Hani A. Tibbo, Markos Adney, Danielle R. Guitian, Javier Amarin, Nadim Richt, Juergen A. McDowell, Chester Steel, John Abu-Basha, Ehab A. Al-Majali, Ahmad M. van Doremalen, Neeltje Munster, Vincent J. Viruses Communication Middle East respiratory syndrome-related coronavirus (MERS-CoV) is a persistent zoonotic pathogen with frequent spillover from dromedary camels to humans in the Arabian Peninsula, resulting in limited outbreaks of MERS with a high case-fatality rate. Full genome sequence data from camel-derived MERS-CoV variants show diverse lineages circulating in domestic camels with frequent recombination. More than 90% of the available full MERS-CoV genome sequences derived from camels are from just two countries, the Kingdom of Saudi Arabia (KSA) and United Arab Emirates (UAE). In this study, we employ a novel method to amplify and sequence the partial MERS-CoV genome with high sensitivity from nasal swabs of infected camels. We recovered more than 99% of the MERS-CoV genome from field-collected samples with greater than 500 TCID(50) equivalent per nasal swab from camel herds sampled in Jordan in May 2016. Our subsequent analyses of 14 camel-derived MERS-CoV genomes show a striking lack of genetic diversity circulating in Jordan camels relative to MERS-CoV genome sequences derived from large camel markets in KSA and UAE. The low genetic diversity detected in Jordan camels during our study is consistent with a lack of endemic circulation in these camel herds and reflective of data from MERS outbreaks in humans dominated by nosocomial transmission following a single introduction as reported during the 2015 MERS outbreak in South Korea. Our data suggest transmission of MERS-CoV among two camel herds in Jordan in 2016 following a single introduction event. MDPI 2021-03-31 /pmc/articles/PMC8067259/ /pubmed/33807288 http://dx.doi.org/10.3390/v13040592 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Seifert, Stephanie N.
Schulz, Jonathan E.
Ricklefs, Stacy
Letko, Michael
Yabba, Elangeni
Hijazeen, Zaidoun S.
Holloway, Peter
Al-Omari, Bilal
Talafha, Hani A.
Tibbo, Markos
Adney, Danielle R.
Guitian, Javier
Amarin, Nadim
Richt, Juergen A.
McDowell, Chester
Steel, John
Abu-Basha, Ehab A.
Al-Majali, Ahmad M.
van Doremalen, Neeltje
Munster, Vincent J.
Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title_full Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title_fullStr Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title_full_unstemmed Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title_short Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
title_sort limited genetic diversity detected in middle east respiratory syndrome-related coronavirus variants circulating in dromedary camels in jordan
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067259/
https://www.ncbi.nlm.nih.gov/pubmed/33807288
http://dx.doi.org/10.3390/v13040592
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