Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

SIMPLE SUMMARY: This study aimed to evaluate the growth, survival rate, and resistance to Acute hepatopancreatic Necrosis Disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. The result showed that shrimps were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum have an effective to increased shrimp growth, stimulated non-specific immune system such as total hemocyte cells, granulocyte cells, hyaline cells, and protected shrimp to ANPND. The supply of CNP to the water have increased the intensity of V. parahaemolyticus effects on shrimp health; significantly decreased non-specific immune parameters of shrimp by 30–50%, therefore increased the AHPND infected rate and mortality of shrimp compared with without CNP group. In summary, LAB has a good effect to shrimp and the supply of CNP had significantly reduced the shrimp’s immune response and increased the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets. ABSTRACT: This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.