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Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles

Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively u...

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Autores principales: Alfassam, Haya A., Nassar, Majed S., Almusaynid, Manal M., Khalifah, Bashayer A., Alshahrani, Abdullah S., Almughem, Fahad A., Alshehri, Abdullah A., Alawad, Majed O., Massadeh, Salam, Alaamery, Manal, Aldeailej, Ibrahim M., Alamri, Aref A., Binjomah, Abdulwahab Z., Tawfik, Essam A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067458/
https://www.ncbi.nlm.nih.gov/pubmed/33917625
http://dx.doi.org/10.3390/pharmaceutics13040502
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author Alfassam, Haya A.
Nassar, Majed S.
Almusaynid, Manal M.
Khalifah, Bashayer A.
Alshahrani, Abdullah S.
Almughem, Fahad A.
Alshehri, Abdullah A.
Alawad, Majed O.
Massadeh, Salam
Alaamery, Manal
Aldeailej, Ibrahim M.
Alamri, Aref A.
Binjomah, Abdulwahab Z.
Tawfik, Essam A.
author_facet Alfassam, Haya A.
Nassar, Majed S.
Almusaynid, Manal M.
Khalifah, Bashayer A.
Alshahrani, Abdullah S.
Almughem, Fahad A.
Alshehri, Abdullah A.
Alawad, Majed O.
Massadeh, Salam
Alaamery, Manal
Aldeailej, Ibrahim M.
Alamri, Aref A.
Binjomah, Abdulwahab Z.
Tawfik, Essam A.
author_sort Alfassam, Haya A.
collection PubMed
description Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as ‘surface plasmonic resonance’ in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients’ swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (−30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients’ swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention.
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spelling pubmed-80674582021-04-25 Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles Alfassam, Haya A. Nassar, Majed S. Almusaynid, Manal M. Khalifah, Bashayer A. Alshahrani, Abdullah S. Almughem, Fahad A. Alshehri, Abdullah A. Alawad, Majed O. Massadeh, Salam Alaamery, Manal Aldeailej, Ibrahim M. Alamri, Aref A. Binjomah, Abdulwahab Z. Tawfik, Essam A. Pharmaceutics Article Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as ‘surface plasmonic resonance’ in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients’ swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (−30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients’ swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention. MDPI 2021-04-06 /pmc/articles/PMC8067458/ /pubmed/33917625 http://dx.doi.org/10.3390/pharmaceutics13040502 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alfassam, Haya A.
Nassar, Majed S.
Almusaynid, Manal M.
Khalifah, Bashayer A.
Alshahrani, Abdullah S.
Almughem, Fahad A.
Alshehri, Abdullah A.
Alawad, Majed O.
Massadeh, Salam
Alaamery, Manal
Aldeailej, Ibrahim M.
Alamri, Aref A.
Binjomah, Abdulwahab Z.
Tawfik, Essam A.
Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title_full Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title_fullStr Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title_full_unstemmed Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title_short Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
title_sort development of a colorimetric tool for sars-cov-2 and other respiratory viruses detection using sialic acid fabricated gold nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067458/
https://www.ncbi.nlm.nih.gov/pubmed/33917625
http://dx.doi.org/10.3390/pharmaceutics13040502
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