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Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory
Innate immune cells such as monocytes and macrophages are activated in response to microbial and other challenges and mount an inflammatory defensive response. Exposed cells develop the so-called innate memory, which allows them to react differently to a subsequent challenge, aiming at better protec...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067467/ https://www.ncbi.nlm.nih.gov/pubmed/33917456 http://dx.doi.org/10.3390/nano11040931 |
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author | Barbosa, Mayra M. Ferrari Kanno, Alex Issamu Farias, Leonardo Paiva Madej, Mariusz Sipos, Gergö Sbrana, Silverio Romani, Luigina Boraschi, Diana Leite, Luciana C. C. Italiani, Paola |
author_facet | Barbosa, Mayra M. Ferrari Kanno, Alex Issamu Farias, Leonardo Paiva Madej, Mariusz Sipos, Gergö Sbrana, Silverio Romani, Luigina Boraschi, Diana Leite, Luciana C. C. Italiani, Paola |
author_sort | Barbosa, Mayra M. Ferrari |
collection | PubMed |
description | Innate immune cells such as monocytes and macrophages are activated in response to microbial and other challenges and mount an inflammatory defensive response. Exposed cells develop the so-called innate memory, which allows them to react differently to a subsequent challenge, aiming at better protection. In this study, using human primary monocytes in vitro, we have assessed the memory-inducing capacity of two antigenic molecules of Schistosoma mansoni in soluble form compared to the same molecules coupled to outer membrane vesicles of Neisseria lactamica. The results show that particulate challenges are much more efficient than soluble molecules in inducing innate memory, which is measured as the production of inflammatory and anti-inflammatory cytokines (TNFα, IL-6, IL-10). Controls run with LPS from Klebsiella pneumoniae compared to the whole bacteria show that while LPS alone has strong memory-inducing capacity, the entire bacteria are more efficient. These data suggest that microbial antigens that are unable to induce innate immune activation can nevertheless participate in innate activation and memory when in a particulate form, which is a notion that supports the use of nanoparticulate antigens in vaccination strategies for achieving adjuvant-like effects of innate activation as well as priming for improved reactivity to future challenges. |
format | Online Article Text |
id | pubmed-8067467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80674672021-04-25 Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory Barbosa, Mayra M. Ferrari Kanno, Alex Issamu Farias, Leonardo Paiva Madej, Mariusz Sipos, Gergö Sbrana, Silverio Romani, Luigina Boraschi, Diana Leite, Luciana C. C. Italiani, Paola Nanomaterials (Basel) Article Innate immune cells such as monocytes and macrophages are activated in response to microbial and other challenges and mount an inflammatory defensive response. Exposed cells develop the so-called innate memory, which allows them to react differently to a subsequent challenge, aiming at better protection. In this study, using human primary monocytes in vitro, we have assessed the memory-inducing capacity of two antigenic molecules of Schistosoma mansoni in soluble form compared to the same molecules coupled to outer membrane vesicles of Neisseria lactamica. The results show that particulate challenges are much more efficient than soluble molecules in inducing innate memory, which is measured as the production of inflammatory and anti-inflammatory cytokines (TNFα, IL-6, IL-10). Controls run with LPS from Klebsiella pneumoniae compared to the whole bacteria show that while LPS alone has strong memory-inducing capacity, the entire bacteria are more efficient. These data suggest that microbial antigens that are unable to induce innate immune activation can nevertheless participate in innate activation and memory when in a particulate form, which is a notion that supports the use of nanoparticulate antigens in vaccination strategies for achieving adjuvant-like effects of innate activation as well as priming for improved reactivity to future challenges. MDPI 2021-04-06 /pmc/articles/PMC8067467/ /pubmed/33917456 http://dx.doi.org/10.3390/nano11040931 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barbosa, Mayra M. Ferrari Kanno, Alex Issamu Farias, Leonardo Paiva Madej, Mariusz Sipos, Gergö Sbrana, Silverio Romani, Luigina Boraschi, Diana Leite, Luciana C. C. Italiani, Paola Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title | Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title_full | Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title_fullStr | Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title_full_unstemmed | Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title_short | Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory |
title_sort | primary and memory response of human monocytes to vaccines: role of nanoparticulate antigens in inducing innate memory |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067467/ https://www.ncbi.nlm.nih.gov/pubmed/33917456 http://dx.doi.org/10.3390/nano11040931 |
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